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Why Cytochrome-c-oxidase is more difficult to measure with near-infrared spectroscopy than hemoglobin? (lpt) 5.

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The enzyme contains four redox active metal centres; one of these, the binuclear CuA centre, has a strong absorbance in the near-infrared that enables it to be detectable in vivo by near-infrared spectroscopy. But concentration of this centre is very less, 10% of the haemoglobin, which makes it harder to detect.

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