Question

P11 Studies of the binding of ATP to the enzyme tetrahydrofolate synthetase were conducted at T-293 K and appear in the following table, where CTp is in molar units. 0.25 0.50 1.0 1.5 2.0 2.5 6.67 X 1.43 X 3.33 x 6.00 x 1.00x 1.67x 3X (M) 10-6 10-5 10-510 10-4 10-4 10-4 a. From a Scatchard plot, determine K293 and N. b. Assume K293 K310 . Using the information from the Scatchard plot of part (a), calculate the standard enthalpy Construct AHo for the binding of ATP to tetrahydrofolate synthetase. Assume AH is constant between T 293 K P18 It i and T 310 K. the bindi Equation

Answer 1