Calculate turnover number if Vmax = 0.681 mMmin-1 assuming that 1 x 10^-4 mol of enzyme was used
The book says the answer is 20.43, but I got 1.61. I used Vmax/Km (Km=0.421), but evidently that's wrong. Any help would be great! Thank you!
Calculate turnover number if Vmax = 0.681 mMmin-1 assuming that 1 x 10^-4 mol of enzyme...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
Need help with number 13! I already asked about number 12. The inverse velocity and inverse substrate concentration relationship for an enzyme-catalyzed reaction is given below V Vmax Vmax S For the hydration of CO2 catalyzed by carbonic anhydrase, it was determined experimentally that (dm s mol 4023.9+ 39.934 at a total enzyme IS] concentration of 2.32 × 10-y mol-dm- What is the value of the Michaelis constant KM for this enzymatic reaction? (B). 9.92x103 mol dm3 (D). 100.8 mol...
An enzyme has a Km for substrate of 10 mM and Vmax of 5 mol L-1 sec-1 at a total enzyme concentration of 1 nM. At [S] = 10 mM, kcat is: A) 2500 per M per sec. B) 5000 per M per sec. C) 1250 per M per sec. D) 2500 per sec. E) 5000 per sec.
help with these please The turnover number is defined as the maximum number of substrate molecules that can be converted into product molecules per unit time by an enzyme molecule. The concentration of enzyme active sites is not necessarily equal to the concentration of enzyme molecules, because some enzyme molecules have more than one active site. If the enzyme molecule has one active site, the turnover number is given by turnover number = - -=k2 (Rmax is often written as...
I have already calculate the catalytic efficieny. Please calculate the Vmax and show work. 1. (10 points) From a kinetics experiment, keat was determined to be 195sec". For the kinetic assay, 0.3mL of a 0.35mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 75,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (UM'min') for the enzyme and catalytic efficiency in M'sec ) for the enzyme. The Kn for the enzyme was determined to be...
Suppose that the following data are obtained for an enzyme-catalyzed reaction: [S] (mM) V (mmol ml-1min-1) 0.1 3.33 0.2 5.00 0.5 7.14 0.8 8.0 1.0 8.33 2.0 9.09 a.) From a double-reciprocal plot of the data, determine Km and Vmax. b.) Assuming that the enzyme present in the system had a concentration of 10-6 M, calculate its turnover number.
A number of inhibitors for studyase have been found, the following data from enzyme assay analysis has been determined: S (mM) Inhibitor A V(μmole/mL/sec) 1mL of 1mM used Inhibitor B V(μmole/mL/sec) 1mL of 3mM used Inhibitor C V(μmole/mL/sec) 1mL of 0.05mM used 1 4.3 5.5 5 2 8 9 8.69 4 14 13 13.7 8 21 16 19.6 12 26 18 22.2 Plot a Lineweaver-Burk plot of 1/V versus 1/S (on 1 graph) of each of the inhibitors. Determine...
Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as β-lactamase), an enzyme present in some resistant bacteria. The amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10-13 moles of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay. I got the right answers for the first two parts but I can't figure out how to find the...
Can anyone help with parts D and E? Ibuprofen [a-methyl-4-(isobutyl)phenylacetic acid; Mr 206] reversibly inhibits cyclo-oxygenase (COX-2), a Michaelis enzyme that converts arachidonate to prostaglandin G2 (PPG2) thereby preventing the fever, inflammation and pain induced by prostaglandins. The following table shows a kinetic analysis of COX-2 in the absence and presence of 10 mg/ml Ibuprofen [Arachidonate] Vo mM/min w/o Ibuprofen Vo, mM/min w/ Ibuprofen mM 0.5 23.5 16.67 25.25 30.49 1.0 32.2 1.5 36.9 2.5 41.8 37.04 38.91 3.5 44.0...
1. A chemical reaction "A" has a DG of -10 kcal/mole. Calculate the key for the reaction A. Show the equation that you have used and all the calculations to get full credit. (2 pts). 2. State two common features among most of the activate carriers that you have studied in class. (2 points) 3. Study the sequence of the peptide given below and answer the following questions based on the peptide sequence given below. AGLRHTICHMKDCFY (10 points) A. Name...