An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene.
An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene. The cloned promoter and coding regions were ligated to luciferase, a reporter gene. Several regions of DNA in the promoter region were deleted. Each construct was transformed into cells, and the luciferase activity was measured. The data from the experiment is listed in the table.
Which conclusions can be drawn from the data?
-Transcription increases when region A is deleted because the promoter region is located in region A.
-Silencers bind repressors and are located in region C.
-Region D does not contain any response elements.
-Transcription binding sites are not found in region B.
-Basal promoters are located in region D because gene expression decreases when it is deleted.
Homework Answers
Transcription binding sites are not found in region B.
Basal promoters are located in region D because gene expression decreases when it is deleted.
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An experiment was conducted to determine the effects of a novel promoter on the transcription of a gene.
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
If a gene is located in a chromatin region that has high levels of histone acetylation......
If a gene is located in a chromatin region that has high levels of histone acetylation... Select one: a. The gene will have a higher chance of being transcribed as chromatin will be more relaxed (open) b. the gene will be expressed at a higher level because repressors will have a lower chance of binding to the promoter of the gene a c. The gene will be less transcribed because repressors will have a higher chance to bind to the...
Which of the following is NOT a function of transcription that requires the activity from subunits...
Which of the following is NOT a function of transcription that requires the activity from subunits of the Core RNA Palymerase? a. RNA polymerase activity that base-pairs and polymerizes nucleotides to make mRNA. b. Helicase activity that unwinds the double-stranded DNA molecule for transcription c. Specific recognition of -35 box and -10 box sites in the promoter region. d. General binding that helps RNA polymerase loosely adhere to DNA, before Transcription begins. Oe. Trick Question. The Core RNA polymerase can...
You are conducting an experiment identifying enhancers that regulate the expression of a gene that codifies...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the...
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
An experiment was conducted in which sections of a eukaryote’s DNA were deleted to see the...
An experiment was conducted in which sections of a eukaryote’s DNA were deleted to see the impact on mRNA production. In each case, transcription was measured by looking at the number of transcripts produced after the gene expression was induced. Given the data below, explain why each of the deletions has the impact it does (or does not). For reference, a wild-type cell (no deletions) produced 15,000 transcripts in this experiment. Region deleted (all regions are upstream of the transcription...
1. trans-acting factors are able to regulate target genes from any chromosome, whereas cis-acting elements can...
1. trans-acting factors are able to regulate target genes from any chromosome, whereas cis-acting elements can only regulate genes located in the same chromosome. a. True only in operons. In Eukaryotic systems, trans-acting factors only regulate genes in the same chromosome. b. True only in Eukaryotes. Prokaryotes don’t have cis-acting elements. c. True for any organism. d. False. The statement is erroneous 2. Unlike activators, repressors never affect chromatin structure. Repressors inhibit transcription only by binding to the binding sites...
I need help with part B. This was the entire problem with all given information. 16....
I need help with part B. This was the entire problem with all
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16. Suppose we want to locate upstream control regions in a certain gene. We have used an exonuclease to chew the gene from the 5 end. Below is the result of several runs, where we have let the exonuclease chew for different periods of time. 1 2 3 4 5 Experiment 1, no exonuclease exposure Experiment 2, 5 min. exonuclease exposure Experiment 3, 10 min....
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
If a gene is located in a chromatin region that has high levels of histone acetylation... Select one: a. The gene will have a higher chance of being transcribed as chromatin will be more relaxed (open) b. the gene will be expressed at a higher level because repressors will have a lower chance of binding to the promoter of the gene a c. The gene will be less transcribed because repressors will have a higher chance to bind to the...
Which of the following is NOT a function of transcription that requires the activity from subunits of the Core RNA Palymerase? a. RNA polymerase activity that base-pairs and polymerizes nucleotides to make mRNA. b. Helicase activity that unwinds the double-stranded DNA molecule for transcription c. Specific recognition of -35 box and -10 box sites in the promoter region. d. General binding that helps RNA polymerase loosely adhere to DNA, before Transcription begins. Oe. Trick Question. The Core RNA polymerase can...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
3) Gene Control in Bacteria - Transcription Activators and Repressors You are interested in examining the regulation of the gene that encodes an enzyme, Tre- ase, important in metabolizing trehalose into glucose in bacteria. Trehalose is a disaccharide formed of two glucose units. It is known that two DNA-binding proteins, TreA and TreB, are important for binding to the promoter of the Tre-ase gene and are involved in regulating the transcription of the Tre-ase gene: TreA binds to the "A"...
I need help with part B. This was the entire problem with all
given information.
16. Suppose we want to locate upstream control regions in a certain gene. We have used an exonuclease to chew the gene from the 5 end. Below is the result of several runs, where we have let the exonuclease chew for different periods of time. 1 2 3 4 5 Experiment 1, no exonuclease exposure Experiment 2, 5 min. exonuclease exposure Experiment 3, 10 min....
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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