Concentration |
Volume used |
Amount of material |
15mM |
45 ul |
|
200uM |
1 ml |
|
250mM |
15 ul |
|
|
125 ul |
200 nMols |
|
9 ml |
25 mMols |
|
10 ul |
5 uMols |
1M |
|
55 uMols |
50uM |
|
100 pMols |
80mM |
|
165 uMols |
Part 1: The amounts of substance in each solution are calculated:
amount = M * V = 15 mmol / L * 10 uL * (1 L / 1x10 ^ -6 uL) = 1.5x10 ^ 8 mmol
2: 0.2 umol
3: 3.75x10 ^ 9 mmol
Part 2: The concentrations are calculated:
Concentrations = n / V = (200 nmol / 125 uL) * (1x10 ^ -6 uL / 1 L) = 1.6x10 ^ 6 nM
5: 2.78 M
6: 0.5 M
Part 3: The volumes are calculated:
Volumes = n / M = 55x10 ^ -6 mol / 1 M = 5.5x10 ^ -5 L
8: 2 L
9: 2x10 ^ -5 L
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