If you were to use a column twice the length of the one that you will...
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If
you were to use a column twice the length of the one that you will...
4. The compounds in a mixture separated by GC can be identified by their retention times....
4. The compounds in a mixture separated by GC can be identified by their retention times. If you Suructor nad not told you which peak in the GC chromatogram corresponded to which alker you didn't know the boiling points of the alkenes, how could you determine the identity or each peak by its retention time? Identities of the components in a mixture separated by GC can also be determined by the technique called "spiking". Suppose you had run a chromatogram...
for HPLC list 3 parameters of the column that you could change if you were choosing...
for HPLC list 3 parameters of the column that you could change if you were choosing a whole new column to improve isocratic seperation of two peaks that were overlapping in hplc. note that flow rate is not a property of the column
What length of column would be required to just resolve two peaks with retention times of...
What length of column would be required to just resolve two peaks with retention times of 92 and 107 s, respectively, using a column with an Hof 1.60 cm/plate?
What would happen to the retention time of a compound if the following changes were made?...
What would happen to the retention time of a compound if the following changes were made? Decrease the flow rate of the carrier gas Increase the temperature of the column Increase the length of the column How do the temperature and flow rate of the carrier gas affect the separation of two compounds that have been shown to separate by GC? 5. Rank the following compounds in terms of expected retention times on a non-polar GC Column TOH OCH.CH MW-114...
25-17. (a) Nonpolar aromatic compounds were separated by HPLC on an octadecyl (CD bonded phase. The...
25-17. (a) Nonpolar aromatic compounds were separated by HPLC on an octadecyl (CD bonded phase. The eluent was 65 vol% meth- anol in water. How would the retention times be affected if 90% methanol were used instead? (b) Octanoic acid and 1-aminooctane were passed through the same column described in (a), using an eluent of 20% metha- nol/80% buffer (pH 3.0). State which compound is expected to be eluted first and why Octanoic acid CH CH CH CH,CH CH,CH CH2NH2...
1. Explain how a fractionating column increases the separation of two liquids. 2. What is an...
1. Explain how a fractionating column increases the separation of two liquids. 2. What is an azeotrope? Do you think the mixture used in the lab is an example of an azeotrope? Explain. 3. Explain how the boiling point of a liquid is affected by the addition of a non-volatile solute (hint: it is a colligative property). Is the temperature above such solution lower, higher or the same as the pure liquid? Explain why. 4. You were asked to record...
You perform a biuret test, letting the samples stand for 10 minutes. How do you think...
You perform a biuret test, letting the samples stand for 10 minutes. How do you think the results may be affected? A The results will not be affected. The reagent reacts instantly. B Ten minutes is less than the required reaction time, so the color will not be as dark. This means that you will detect less analyte than is actually in the sample. C Ten minutes is less than the required reaction time, so the color will not be...
A number of factors determine the rate at which a given compound travels through a gas chromatograph. List two of t...
A number of factors determine the rate at which a given compound travels through a gas chromatograph. List two of these factors: The period following injection that is required for a compound to pass through the column is called the What would happen to the retention time of a compound if the following changes were made? Decrease the flow rate of the carrier gas Increase the temperature of the column Increase the length of the column How do the temperature...
When you want a loop to iterate exactly n times, you will typically use one of...
When you want a loop to iterate exactly n times, you will typically use one of two standard for loops patterns (see p. 95-96): for (int i = 1; i <= n; i++) for (int i = 0; i < n; i++) Although both of these for loops will output n times, they have different initialization and test conditions. Please give two *examples to describe why it is beneficial to have both patterns. (i.e. think of the initialization condition and...
Please use your own experiences and the knowledge you have gained from this week’s readings to...
Please use your own experiences and the knowledge you have gained from this week’s readings to answer the following topics and questions. You may also use information that you find in the textbook, AIU’s library or the Internet to support your discussion. Make sure you use economic concepts in your main contribution. In your contribution to this week’s Discussion Board, you will discuss how you are affected by unemployment, one of the country's biggest macroeconomic policy concerns. Address both of...
4. The compounds in a mixture separated by GC can be identified by their retention times. If you Suructor nad not told you which peak in the GC chromatogram corresponded to which alker you didn't know the boiling points of the alkenes, how could you determine the identity or each peak by its retention time? Identities of the components in a mixture separated by GC can also be determined by the technique called "spiking". Suppose you had run a chromatogram...
What would happen to the retention time of a compound if the following changes were made? Decrease the flow rate of the carrier gas Increase the temperature of the column Increase the length of the column How do the temperature and flow rate of the carrier gas affect the separation of two compounds that have been shown to separate by GC? 5. Rank the following compounds in terms of expected retention times on a non-polar GC Column TOH OCH.CH MW-114...
25-17. (a) Nonpolar aromatic compounds were separated by HPLC on an octadecyl (CD bonded phase. The eluent was 65 vol% meth- anol in water. How would the retention times be affected if 90% methanol were used instead? (b) Octanoic acid and 1-aminooctane were passed through the same column described in (a), using an eluent of 20% metha- nol/80% buffer (pH 3.0). State which compound is expected to be eluted first and why Octanoic acid CH CH CH CH,CH CH,CH CH2NH2...
A number of factors determine the rate at which a given compound travels through a gas chromatograph. List two of these factors: The period following injection that is required for a compound to pass through the column is called the What would happen to the retention time of a compound if the following changes were made? Decrease the flow rate of the carrier gas Increase the temperature of the column Increase the length of the column How do the temperature...
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