You buy a protein standard at a concentration of 4mg/200ml. How would you make 1 ml of a 10ug/ml, working solution?
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9. (4 pts) Trifluoroacetic acid (CF3CO2H; pk, = 0.23) and acetic acid (CH3CO2H; pka = 4.75) are monoprotic acids (meaning they can dissociate only one H+ ion/molecule of acid). Using your knowledge of structure, explain why the acid dissociation (Ka) of acetic acid is greater than that for trifluoroacetic acid. Place your answer in the space provided. indisht n.....
Acetic acid (CH3COOH) and trifluoroacetic acid (CF3COOH) are shown by these particulate models. The K, for trifluoroacetic acid is 104times greater than acetic acid. Use the models to account for the differences in magnitude for the K, constant. The yellow atoms are fluorine, the red atoms are oxygen, the black atoms are carbon, and the white atoms are hydrogen. Part 1Write the formulas for the conjugate bases for the two structures The conjugate base of acetic acidThe conjugate base of trifluoroacetic acid Part...
Do the calculations to make 200ml of 0.1M sodium acetate buffer with a pH of 5.1 starting with 5.0M acetic acid and 1.0M NaOH ****show calculations***** 1.How much acetic acid is needed? 2.What is the ratio of Ac- to HAc at pH of 5.1? 3. What fraction of total acetate is Ac-at pH 5.1 4. How much OH- is needed to obtain 68.7% Ac-?
show how you would make this compound...
2. Show how you would make this compound by a malonic ester synthesis or an acetoacetic acid synthesis. 4-methylhexanoic acid
Acetic acid (CH:COOH) and trifluoroacetic acid (CF COOH) are shown by these particulate models. The K, for trifluoroacetic acid is 10 times greater than acetic acid. Use the models to account for the differences in magnitude for the K, constant. The yellow atoms are fluorine, the red atoms are oxygen, the black atoms are carbon, and the white atoms are hydrogen. 1st attempt Part 1 (2 points) Write the formulas for the conjugate bases for the two structures. The conjugate...
Acetic acid (CH3COOH) and trifluoroacetic acid (CF3COOH) are shown by these particulate models. The Ka for trifluoroacetic acid is 104 times greater than acetic acid. Use the models to account for the differences in magnitude for the Ka constant. The yellow atoms are fluorine, the red atoms are oxygen, the black atoms are carbon, and the white atoms are hydrogen.Part 1Write the formulas for the conjugate bases for the two structures. The conjugate base of acetic acid Part 2 Which of the following...
A reminder of the henderson- equation Do the calculation to make 200ml, of 0.1M sodium aculate buffer with a pathe of 5.1 with 5 xm acis and 1 CM NaCH. Calculate the total amount of acetric acid needed. Calculate the radio of the two form of that will when the pH is 5.1 Use this ratio to calculate the % of calculate that will be in the A form. Assume that each NaOH will convert one. Use this plus the...
"H NMR spectrum of 5,10,15,20-Tetraphenylporphyrin (major isomer dissolved in deuterated chloroform with 1% trifluoroacetic acid.) For the assignment, draw the structure and we a letter from the table to deate the set of protons responsible each signal. Report the chemical shift value to the carest 0.01 Attach the spectrum lo the repor chemical shift multiplicity Integration ratio Assignment Structure with assigned protons: 2 CFzco HH excess Hi fr 'H NMR spectrum interpretation Score. INMR spectrum of 5,10,15,20-Tetraphenylporphyrin (dissolved in CDCl,...
What should the LOD score be when RF = 0.5? How would you make some biological/genetic sense of the result? RF means recombination frequency.