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For TLC plates of amino acids: You will be using a mixture of 5:1:4 volume ratio...

For TLC plates of amino acids:

You will be using a mixture of 5:1:4 volume ratio of butanol, glacial acetic acid (pure acetic acid), and water, pH of water is 7, in this lab.

Assume that butanol is the same as water in terms of a pH calculation.

The density of acetic acid is 1.05 g/ml.

The molecular weight of acetic acid is 60.05 g/mol.

The Ka of acetic acid HAc is 1.8*10^-5.

You can make the assumption that [H+] = [Ac-] << [HAc] here.

What is the pH of this solution?

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