You’re studying proteins and the effect of pH on those proteins. For that purpose you are...
You’re studying proteins and the effect of pH on those proteins. For that purpose you are going to make two buffers. First, how would you prepare a 2L of a 10 mM Phosphate buffer at pH of 7.4?
You have –
Solid KH2PO4
Solid K2HPO4
10M KOH (as much as you need)
purified water
pH meter
balance, glassware and anything else you need.
The three pKa values for phosphoric acid (from the CRC Handbook of Chemistry and Physics) are 2.16, 7.21, and 12.32.
(Note: there is more than one way to do this and you don’t necessarily have to use all of the above.)
Then pick a second buffering agent to use at a different pH. It must be at least 2 pH units away from the buffer above. Explain how you would prepare 2L of 10mM of that buffer as well.
Homework Answers
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I added everything thing. this is the lab question you need to solve. First assigned buffer...
I added everything thing.
this is the lab question you need to solve.
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1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2....
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please help with my pre lab additional information Pre-Lab Questions: 1. What is the definition of...
please help with my pre lab
additional information
Pre-Lab Questions: 1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCI stock solution. In your response to this question, be very specific about the quantities of stock solution and...
3. If 15.0 mL of 0.125 M phosphoric acid is titrated with 0.100 M NaOH, what...
3. If 15.0 mL of 0.125 M phosphoric acid is titrated with 0.100 M NaOH, what volume of the titrant (in mL) must be added to completely neutralize the acid? Show all of your work (including the chemical equation). (1 point) Post-lab Questions: Experiment #9: Acid-Base Titrations Student Learning Objectives : Students will gain practice with the accurate preparation of solutions. Students will perform acid-base titrations and prepare titration curves. Students will identify strong and weak acids by the shapes...
Cabrillo College Chemistry 1B Buffer Problem Set 1. A desires study the effect of pH n the growth of E. coli bacteria. She plans to do the research by preparing culture media that are buffered at three distinctly different PH values. Therefore, she decides to use the triprotic phosphoric acid (H,PO), potassium dihydrogen hydrogen phosphate o") and potassiam phosphate POe) to prepare the buffers K-1 7.11 103 Ka- 6.32 x 10" Kal 7.11 x 10 MATERIALSAFALLABLE Standardized solutions: 1.00 MH3PO,...
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I added everything thing.
this is the lab question you need to solve.
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1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
please help with my pre lab
additional information
Pre-Lab Questions: 1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCI stock solution. In your response to this question, be very specific about the quantities of stock solution and...
3. If 15.0 mL of 0.125 M phosphoric acid is titrated with 0.100 M NaOH, what volume of the titrant (in mL) must be added to completely neutralize the acid? Show all of your work (including the chemical equation). (1 point) Post-lab Questions: Experiment #9: Acid-Base Titrations Student Learning Objectives : Students will gain practice with the accurate preparation of solutions. Students will perform acid-base titrations and prepare titration curves. Students will identify strong and weak acids by the shapes...
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