Question

A drug D binds to a protein P with binding constant K1 = 1.58x10^6 at temperature T1 = 298 K and with binding constant K2 = 1x10^6 at temperature T1 = 330 K. Determine how the extent of the binding of the drug θ changes if the analytical concentration of the drug [D]0 = 50 pM is held constant as the temperature is raised from 273 K to 373 K. You can assume that the temperature dependence of the binding constant over this range of temperatures is linear with respect to temperature for ln K.

Answer 1