Question

(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points) Arrange the proteins in the order of elution in gel permeation chromatography. List the first one clutes first. Use the letters provided ii) (5 points) Which protein/'s will be eluted from a cation exchange column at pH5? Use the letters provided. iv) (5 points) Which protein's will be stuck in an anion exchange column at pH 7? Use the letters provided v) (5 points) List thei of following reagents in gel electrophoresis Urea Beta-

Answer 1

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