Question

1) Acetic acid is produced using bacteria in batch culture. It was shown that for each mole of glucose utilized by the bacteria, 3.60 moles of cells, 1.10 moles of acetic acid, 1.30 moles of gaseous CO2, and some H20 are formed. Oxygen and ammonia were consumed. Other components of the system such as phosphate can be neglected. Cellular composition of bacteria might be taken as CH1.770049No.24 Medium containing glucose and ammonia dissolved in 15,000 litres of water is pumped into a stirred bioreactor and inoculated with the bacteria. Air is sparged into the bioreactor, the total amount of off-gas recovered during the entire batch culture is 950 kg. The final acetic acid concentration should not be allowed to exceed 5 wt%. How much glucose and ammonia are required? What is the percentage of gases in the off-gas? a. b. 2) For the above described set-up, when the bacteria is grown at 37°C, estimate the cooling requirements

Answer 1

Answer;

1) a) this question simply is the one related to mole concept. Since 1 mole of CO₂ means 44 g so 1.30 moles of it means 57.2 g

it is given in the question that 1 mole of glucose is utilised by the bacteria gives 1.30 moles (57.2g) of CO₂ so 950 Kg of gas (let us assume it mainly consist of CO₂ ) will require 16608.39 mole of glucose. And 1 mole of gluccose means 180 g of it so 16608.39 mole of glucose means 2989.5 Kg of it.

b) The percentaage of off-gases is 31.77%.