In gas chromatography, why, even though you injected a mixture containing the same volume of each...
In gas chromatography, why, even though you injected a mixture containing the same volume of each component, the three eluted peaks, in the chromatogram, do not have the same intensity? Mention at least two good reasons for this behavior
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In gas chromatography, why, even though you injected a mixture containing the same volume of each...
9 VII. Gas Chromatography 1. The organic mixture to be separated on the GC is injected...
9 VII. Gas Chromatography 1. The organic mixture to be separated on the GC is injected into the GC as a (gas, liquid, or solid). 2. The typical volume of mixture injected into a GC is 1-10 (microliters, milliliters, or deciliters). 3. Immediately upon entering the injection port the mixture is_ _(solidified, condensed, or vaporized). (heated, cooled, maintained at room 4. The event described in #3 occurs because the injection port is temperature). 5. An inert gas, referred to as...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography?...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Gas Chromatography Analysis: Please interpret the set of GC data below. This set of data consists...
Gas Chromatography Analysis:
Please interpret the set of GC data below. This set of data consists of a mixture of three compounds (3-methylbutan-1-ol, hexanal, and cyclopentanone shown in the lab manual) that have similar boiling points. There is a gas chromatogram of the mixture and gas chromatograms of the individual compounds for peak identification by retention time. You will answer the following questions based on this data set: 1. Was our gas chromatograph able to separate three compounds with similar...
QUESTIONS ON DISTILLATION TO BE TURNED IN Name - You were to separate a mixture containing...
QUESTIONS ON DISTILLATION TO BE TURNED IN Name - You were to separate a mixture containing 20 ml of hexane and 20 ml of sodium acetate. At 60°C, the total pressure of the system is 720 torr. 1. What is the mole fraction of each component? 2. Calculate the partial pressures of the components. 3. What is the vapor pressure lowering of hexane? What other two properties, entities, or functions have similar properties as vapor pressure lowering? What happens as...
2. If you have two beakers of liquid, one containing hexane and one containing water, you...
2. If you have two beakers of liquid, one containing hexane and one containing water, you will notice that one has a smell and the other does not. Explain which one you can smell (using the wafting technique) and why you can smell that one liquid over the other. Discuss the interparticle forces. Use complete sentences. Do not use any abbreviations. 3. For the following pairs, predict which compound would have the higher melting point and explain why by discussing...
You have three solutions each containing the same concentration of a different protein. Two of these...
You have three solutions each containing the same concentration of a different protein. Two of these proteins have 2 Trp in their sequence, whereas the third one only contains one Tyr and one Phe. Which protein solutions will show the highest absorbance at 280nm? Why?
You have a solution containing human myoglobin (16.7 KD, pi7.0), hemoglobin (64.5 KD; pi7.1). and serum...
You have a solution containing human myoglobin (16.7 KD, pi7.0), hemoglobin (64.5 KD; pi7.1). and serum albumin (66,5 KD: pl 4,9). You want to separate these proteins and you run one portion of the solution through anion exchange chromatography at pH 8 and a second portion of the solution through gel filtration. You measure the protein concentration in fractions collected from both columns and you get the following elution profiles Anion Exchange filtration Protein Concentration Eurim Volume Elution Volume Based...
Please answer true or false and explain why for each of them. Thank you 1. Mark...
Please answer true or false and explain why for each of them.
Thank you
1. Mark with T (True) or F (False) (2 points each) • Joule Thompson experiment corresponds to process with constant enthalpy. . The state functions (U.H,G,A) act as thermodynamic potentials when represented as functions of their natural variables. • The Gibbs free energy is equal to the maximum PV work done by the system on the envi- ronment. . The standard enthalpy of formation for any...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Separating a Mixture, Recrystallization, pre-lab assignment could you also explain why you chose that substance for...
Separating a Mixture, Recrystallization, pre-lab
assignment
could you also explain why you chose that substance for the empty
spaces and question marks
EXPERIMENT 4 Pre-Lab Assignment Separating a Mixture, Recrystalliration Name Date 1. Complete the following flowchart which shows how to separate a mixture of sand, sodium chloride and acetanilide. Notice that after a separation process (a down arrow) the filtered solids are shown on the left and the filtrate (the liquid) is shown on the right. The terminal step...
9 VII. Gas Chromatography 1. The organic mixture to be separated on the GC is injected into the GC as a (gas, liquid, or solid). 2. The typical volume of mixture injected into a GC is 1-10 (microliters, milliliters, or deciliters). 3. Immediately upon entering the injection port the mixture is_ _(solidified, condensed, or vaporized). (heated, cooled, maintained at room 4. The event described in #3 occurs because the injection port is temperature). 5. An inert gas, referred to as...
2. If two different compounds have the same Rr value, how might they be identified using paper chromatography? 3. Why is it important to keep the spots on the paper as small as possible? 4. If the solvent pool at the bottom of the beaker touched the spots on the 1.5cm line, what would happen to the spots? How would you address this error? 5. If you were teaching a student how to do paper chromatography, what three pieces of...
Gas Chromatography Analysis:
Please interpret the set of GC data below. This set of data consists of a mixture of three compounds (3-methylbutan-1-ol, hexanal, and cyclopentanone shown in the lab manual) that have similar boiling points. There is a gas chromatogram of the mixture and gas chromatograms of the individual compounds for peak identification by retention time. You will answer the following questions based on this data set: 1. Was our gas chromatograph able to separate three compounds with similar...
QUESTIONS ON DISTILLATION TO BE TURNED IN Name - You were to separate a mixture containing 20 ml of hexane and 20 ml of sodium acetate. At 60°C, the total pressure of the system is 720 torr. 1. What is the mole fraction of each component? 2. Calculate the partial pressures of the components. 3. What is the vapor pressure lowering of hexane? What other two properties, entities, or functions have similar properties as vapor pressure lowering? What happens as...
You have a solution containing human myoglobin (16.7 KD, pi7.0), hemoglobin (64.5 KD; pi7.1). and serum albumin (66,5 KD: pl 4,9). You want to separate these proteins and you run one portion of the solution through anion exchange chromatography at pH 8 and a second portion of the solution through gel filtration. You measure the protein concentration in fractions collected from both columns and you get the following elution profiles Anion Exchange filtration Protein Concentration Eurim Volume Elution Volume Based...
Please answer true or false and explain why for each of them.
Thank you
1. Mark with T (True) or F (False) (2 points each) • Joule Thompson experiment corresponds to process with constant enthalpy. . The state functions (U.H,G,A) act as thermodynamic potentials when represented as functions of their natural variables. • The Gibbs free energy is equal to the maximum PV work done by the system on the envi- ronment. . The standard enthalpy of formation for any...
what is the prediction of the unknown amino acids mixture
based on the strip from thin layer chromatography sheet?
The unknown mixtures can be ala plus asp, ala plus asp plus
lys, lys plus asp or ala plus lys.
anion exchange resin was used
Tubes 2,3, 6 and 7 had a spot appear that was light pink
We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
Separating a Mixture, Recrystallization, pre-lab
assignment
could you also explain why you chose that substance for the empty
spaces and question marks
EXPERIMENT 4 Pre-Lab Assignment Separating a Mixture, Recrystalliration Name Date 1. Complete the following flowchart which shows how to separate a mixture of sand, sodium chloride and acetanilide. Notice that after a separation process (a down arrow) the filtered solids are shown on the left and the filtrate (the liquid) is shown on the right. The terminal step...
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