Question

The Salmonella toxin SpvC catalytically removes one of the phosphates from the activation loop of MAP kinase to produce an inactive MAP kinase. The modified kinase cannot be phosphorylated by any MAP kinase kinase. On the basis of mass spectrometry (which detects the neutral form of phosphate esters), the SpvC-catalyzed dephosphorylation leads to a loss of 98 Da instead of 80 Da expected for hydrolysis. Suggest a plausible structure for the modification induced by SpvC OH P=O SpvC H O O NIL MAPK activtion loop

Answer 1

Solution:

Answer 1-1 но НЗРо О-Р-он 0 NH2 mAPK acRvared loop SPVC IN E H но 3PoL