Question

A set of samples of known concentration were used to create a calibration curve for rubxanthin. The data are shown below. Calibration Curve for Rubixanthin 1.60 1.40 a 1.20 1.00 0.80 0.60 0.40 0.20 0.00 y 114800x R2 0.9999 0.00E+00 2.00E-06 4.0OE-06 6.00E-06 8.00E-06 1.00E-05 1.20E-05 1.40E-05 Concentration (M) 30) An extract of rubixanthin of unknown concentration was analyzed with UV-Vis Spectroscopy. The unknown had an absorbance of 0.83. What is the concentration (M) of rubixanthin in the unknown?

Answer 1

The equation of the line of absorbance versus concentration is given as follows.

Absorbance = 114800*concentration

Now, if the absorbance of unknown = 0.83

Then, 0.83 = 114800*concentration

Therefore, the concentration of rubixanthin in the unknown = 7.23*10-6 M