Question

Question 2: You have just discovered a new enzyme that catalyzes the degradation of cellulose to glucose. You hope to commercialize this enzyme ultimately to invent a process for biofuel manufacture. In order to determine how efficient the enzyme is and how fast the enzyme catalyzes the reaction, you have to perform some basic enzyme kinetics. Plot the following enzyme data for the enzyme catalyzed reaction and determine the Km and Vmax. Show your graph with correct units on the axis and with your answer. (4 points) Enzyme Data for the Enzyme Catalyzed Reaction Substrate (cellulose) Concentration (molar cellulose to glucose ( Rate of conversion from tion) m/min) 0 98 230 390 600 680 700 15 24 50 95 180

Answer 1

Pate ofcomuush en Substhrat 98 230 396 e-0043S 0,067 o.04167 0.00 56 ー 24 So 9S (80 0.0(05 6,o6Ss 680 0,001 0.001488 CS] Vmex

0.012 т y 0.056x +0.0007 R 0.9915 0.01 0.008 (min/micromolar) 0.006 0.004 0.002 0 0 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 1/[S] (1/micromolar)