Question

Blood samples were drawn from five individuals as indicated below (a-e). The RBCs in each sample were removed, then each sample was incubated with fluorescently-labeled antibodies against CD4 and a different fluorescently-labeled antibody against CD8. The samples were then analyzed by flow cytometry. For each individual (a-e), draw a separate dot plot with CD4 fluorescence on the horizontal axis and CD8 fluorescence on the vertical axis. On the dot plot, indicate where you’d expect major cell populations to be located by drawing a cloud of dots to represent each distinct cell population. Identify (i.e. label) the cell types in each major cell population that you draw. Provide a 1-2 sentence explanation for each plot, explaining the effect of the deficiency on the immune system and how that impacted the flow cytometry results you’ve drawn. (10 pts)

a. normal healthy individual

b. patient with IL-2Rg deficiency

c. patient with Rag 2 deficiency

d. patient with AIRE deficiency

e. patient with HLA-DM deficiency

[Assume each deficiency is a complete null]

Answer 1

AIRE u Co 4