Question

If you dissolve 412 mg sucrose (formula weight: 342.29 g/mol) in 60 mL of water:

a. What is the concentration of sucrose in mM in µM?

b. If you take 5 mL of this solution and dilute it to 250 mL, what is the concentration of the glucose in the resulting solution in µM?

c. How many nano moles of sucrose are present in 10 mL of the diluted solution?

Answer 1

1g1mol sucrose 1000 mg 342.29 g a)No of moles of msucrose solute-412 mg × 0.00120 mol sucrose Volume of solution 60 mL 0.060 L no of moles of sucrose Molar concentrati on of sucrose - volume of soln in liters 0.00120 mol 0.060 L -0.0200 mol/L = 0.0200 M × 100 nM 1 M =20mM 106 μΜ 0.0200 MxF-20,000 HM l M b) Concentration of the stock sucrose solution, M,-20,000 μΜ Volume of the stock sucrosesolution, V- 5 mL Volume of the dilutesucrose solution, 250 mL Concentrati on of the dilute sucrose solution, M-? According to dilution principle, 14-YA4 Concentrati on of dilute sucrose soluti on, M,- 11 (5 mL): (20,000 μΜ) 250 mL 400 μ1f 1A2 c) Concentration of the dilute sucrose solution, M,-400 uMX-Ό--0, 0004 M 10°HM Volume of solution = 100 mL = 10 mL = 0.010 L 0.0004 mol sucrose 10° nmol Amount of sucrose solute present 0,010 L × 1L mo 4.0×103 nmol sucrose = 4000 nmol sucrose