A specimen sample was streaked in a plate of agar. Only gram negatives grew on the agar and certain gram negatives resul...
Homework Answers
CLED is a combination of Blood Agar and MacConkey agar combined. Major constituents of CLED agar are Lactose, Pancreatic digest of gelatin and casein, beef extract, cystine, pH indicator Bromothymol blue and Agar. Bromothymol Blue is the pH indicator to differentiate lactose fermenters from lactose-nonfermenters. Organisms capable of fermenting lactose will lower the pH and change colour of the medium from green to yellow.
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A specimen sample was streaked in a plate of agar. Only gram negatives grew on the agar and certain gram negatives resul...
How would you verify that the colonies that grew on a MacConkey agar plate were Gram...
How would you verify that the colonies that grew on a MacConkey agar plate were Gram negative? What types of bacteria are inhibited on MacConkey agar?
a) Will all gram positive organisms grow on a rose agar plate? ecplain why or why...
a) Will all gram positive organisms grow on a rose agar plate? ecplain why or why not. b) Will all gram negative organisms grow on a MAC agar plate? explain why or why not. c) Can colony morphology be done from a MAC or Rose agar plate? why or why not.
1)An eosin methylene blue (EMB) agar plate was inoculated with four different species of bacteria. Below...
1)An eosin methylene blue (EMB) agar plate was inoculated with four different species of bacteria. Below are the colony growth after inoculation. Which shows a vigorous lactose fermenter? A) A brown media with an oblong culture of bacteria. The colony shows vigorous growth and is the same color as the media. B) A brown media with an oblong colony of bacteria that experiences weak growth. The color of the media is visible through the translucent white color of the bacterial...
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
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Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
Microbiology - Gram Positive (ONLY answer) [Q1] A clear zone on blood agar is also known...
Microbiology - Gram Positive (ONLY answer) [Q1] A clear zone on blood agar is also known as _____________ hemolysis. [Q2] Which organism is the only one that can grow in the presence of 6.5% salt? [Q3] What color would you observe for an organism that is negative for mannitol fermentation?
Microbiology - Gram Positive (ONLY answer) [Q1] A positive catalase test shows - Bubbles - Appearance...
Microbiology - Gram Positive (ONLY answer) [Q1] A positive catalase test shows - Bubbles - Appearance of Clots - Change of color from blue to green. - All answers are correct. [Q2] A complete blood cell lysis is also known as - Alpha hemolysis - Beta hemolysis - Gamma hemolysis - No answer text provided. [Q3] How do you know whether your organism completely lysed the blood cells? - By the presence of a green halo around the bacterial growth...
help please On a mannitol-salt agar plate, a bacterium grows on the plate, and turns medium...
help please
On a mannitol-salt agar plate, a bacterium grows on the plate, and turns medium color from light orange to bright yellow. The color indicator is phenol red. This bacterium must be capable of: Select one: O a. Fermenting lactose to make acidic by-products. O b. Utilizing citrate as a main carbohydrate source to grow. O c. Fermenting glucose to make acidic products, which lowers the pH of the MSA agar. O d. Fermenting mannitol to make acidic products,...
Date: Group: Name: Table 2. Bacteria isolated from the skin on the blood agar plate. Colony...
Date: Group: Name: Table 2. Bacteria isolated from the skin on the blood agar plate. Colony type-II Colony type-l Colony type-III Gram stai Colony morphology, margin, elevation, color, etc. Hemolysis Table 3, Bacteria isolated from the rectal area on the EMB plate. Colony type-I Colony type-III Colony type-II Gram stain Colony morphology, margin, elevation, color, etc. Colony arrangement 4. Review questions d. In summary, what is the major purpose of this experiment? What are four reasons for knowing which microorganisms...
E. coli cells were spread on two agar plates [one containing only agar (plate 1) and...
E. coli cells were spread on two agar plates [one containing only agar (plate 1) and the second containing streptomycin (plate 2) which kills all cells except those that are resistant mutants]. Both plates were allowed to grow for several generations. Cells were collected from the agar plate WITHOUT streptomycin and spread again on a fresh agar plate containing streptomycin (plate 3). More mutants were observed on plate 3 than were observed on plate 2. The experiment demonstrates A. how...
10. Fill in the blanks in this diagram to make a key for these 3 bacteria:...
10. Fill in the blanks in this diagram to make a key for these 3 bacteria: Escherichia coli, Enterobacter aerogenes and Staphylococcus aurvens. Growth on EMB No es Metallic green colonies Thick mucoid pink colonies Growth on Mannitol Salt Agar Genus: Gram stain: Genus: Gram stain: Yes with yellow zone around growth Genus: Gram stain: 11. How can one distinguish the 3 common urinary tract pathogens on the HardyCHROMTM UTI demo plate? 12. What is the value of making a...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
help please
On a mannitol-salt agar plate, a bacterium grows on the plate, and turns medium color from light orange to bright yellow. The color indicator is phenol red. This bacterium must be capable of: Select one: O a. Fermenting lactose to make acidic by-products. O b. Utilizing citrate as a main carbohydrate source to grow. O c. Fermenting glucose to make acidic products, which lowers the pH of the MSA agar. O d. Fermenting mannitol to make acidic products,...
Date: Group: Name: Table 2. Bacteria isolated from the skin on the blood agar plate. Colony type-II Colony type-l Colony type-III Gram stai Colony morphology, margin, elevation, color, etc. Hemolysis Table 3, Bacteria isolated from the rectal area on the EMB plate. Colony type-I Colony type-III Colony type-II Gram stain Colony morphology, margin, elevation, color, etc. Colony arrangement 4. Review questions d. In summary, what is the major purpose of this experiment? What are four reasons for knowing which microorganisms...
10. Fill in the blanks in this diagram to make a key for these 3 bacteria: Escherichia coli, Enterobacter aerogenes and Staphylococcus aurvens. Growth on EMB No es Metallic green colonies Thick mucoid pink colonies Growth on Mannitol Salt Agar Genus: Gram stain: Genus: Gram stain: Yes with yellow zone around growth Genus: Gram stain: 11. How can one distinguish the 3 common urinary tract pathogens on the HardyCHROMTM UTI demo plate? 12. What is the value of making a...
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