2)
Average retention time
= [tm(A) + tm(B) +tm(C)]/3
= [2.5 min + 1.8 min + 2.7 min]/3
= 2.33 min (answer)
Resolution between peak B and C
= [tm(C)- tm(B)]/0.5[W(C) + W(B)]
= [2.7 min - 1.8 min]/ 0.5[1.62 min + 1.11 min]
= 0.9 min/1.365 min
= 0.659 (answer)
2. A chromatogram confirms the presence of four components A, B, C are given in the following table. Use this informati...
The table contains data for a liquid chromatography separation of four compounds, A, B, C, and D, on a 40.0 cm column Retention Compound time (min) Width at the base (min) A 5.17 0.436 B 1.02 13.0 15.0 C 1.10 23.4 1.91 Calculate the number of theoretical plates, N, for each peak. NA NB Nc ND = What is the average number of theoretical plates, Navg, for Navg the column? What is the plate height, H, for the column? Н-...
or questions 24-25 answer all the following questions: a)State the Null and Alternate H potheses b) Which is the claim? c) Will you use a z-test, t-test or chi- square test? What is the critical v d) Describe the reject repion as i e) Find the sample statistic 1) Do you reject the claim? Write out your decision in at least one sentence eft tailed, right tailed or two-tailed. 24. The Healthy Food Company makes an Oatmeal and Almond cereal....
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...