Question

(25 pts total) Imagine that the below figure is of Chil-seg data, zoomed in at the genomic locus of the gene, Tumor Suppressor X. Modified from Labrie et al. (2016). Nature Structural & Molecular Biology. 23(6):566-576. [Fig. 2B). _ ._ . L K27ac Signal intensity Do Re H3K4me1 H3K4me3

Answer 1

Answer, In order to test whether the transcript levels of tumor suppressor x vary among the samples, wie intend to perform a RT-PCR (Real time - Quantitative Polymerase chain reaction] for the same we would design a Set of primers. (1) one for APDH ( control gene) (11) one for Tumor supressor X (test gene) - Total RNA would be isolated from the sample using "TRIZOL" method and converted to "CONA" using reverse transcriptase. enzymatic reaction. Syboigreen Taq polymerase is then used to perform a q pcr reaction to Quantify the transcolipt levels of tumor suppressor x in the different Samples.

. We would expect in the tumor cells normal cell sample. its as levels to be compared to lohler the g if can and one knows the target easily design primers perform a chip q PCR. genes. One against them h when targets are not known the DNA fragments pulled down during a chip assay need to be sequenced and hence a chip - seq needs to be used.