Question

The stock of Running Buffer is made up as follows: 15 g Tris base 72 g glycine 50 ml 10% SDS Adjust volume to 5 litres (The mwt of Tris is 121.14; the mwt of glycine is 75.07) Question 4 Answer saved Marked out of 1.00 Flag question What volume of 20% SDS stock will you need, if you are to make up 250 ml of running buffer? Select one: O a. 1.25 ml b. 25 ml c. 50 ml d. 5 ml

Answer 1

Here som 10% sos is diluted to Slt i.e. 5000 mi to make the stock running buffer.. . According to problem since 10% SDS auill be tak en 100m solution contain sos log 50 a 10x50. =59 wow, soome running buffer contain sos 59 Fฐฯ . 100 u sx18! = 0.1 g S08 . Je final wonning buffer sos present 0.1% wow we have to find out what volume of 20% so s stock reauired to make 250 m of 0% sos for wening buffer using a Vale where, & Initial volume = ? c Guitial concuration: 20% Viz final volume = 2som Gr final concentration - 0.4%. : vix 20% - 250m x sih. = = 25 m Vi 1.2 SON in 1.25m of 20% sos needed. Am! (a) HHAHADANI