Question

7. In 1516 the Duke Wilhelm IV of Bavaria introduced the german purity law for brewing beer. More than 500 years later the law is still strictly enforced and it states that for brewing beer you must not use any ingredients other than water, barley and hops. Barley is contributing starch that is broken down to di- and monosaccharides during the fermentation process. Production of some cheap (and ir Germany illegal) beer, however, utilises sucrose as sugar. Using the indicated reagents, describe arn experiment wit sitable controls that allows authorities to determine if a beer was produced using barley or sucrose. Explain the carbohydrate chemistry behind the test to justify your answer. (1 mark) In addition to general laboratory equipment the ONLY reagents available to you are - a beer sample -henzymes invertase and maltase the Somogyi-Nelson reagents for the determination of reducing sugars. il concisely describe the tests that you would use, must be less than 100 words (0.5 0.5 marks) iil concisely describe the observations in case that the beer was made with barley, must be less than 50 words (0.25 marks) ili) concisely describe the observations in case that the beer was made with sucrose must be less than 50 words (0.25 marks

Answer 1

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