Question

whereas dephosphorylation by 12. Phosphorylation of a protein by a requires requires a. Protein kinase, ATPprotein phosphatase, water b. Protein kinase water: protein phosphatase; ATP c. Protein kinase, ATP, protein phosphatase: ADP d. Protein phosphatase: ATP: protein kinase; ADP e. None of the above 13. How many phosphoester and phosphoanhydride bonds are in Z-deoxyadenosine diphosphate? b. 1,2 c.0.1 d. 1,0 6.2.0 14. Give the systematic name of the sugar below Lactose a. a-D-galactopyranoyl-(1.4)--D-glucopyranose b.a-D-glucopyranosyl-(1.4)-1-D-galactopyranose CB-D-glucopyranosyl-(1,5)- -D-glucopyranose d.B-D-galactopyranosyl-(1,5)- G-D-glucopryanose e -D-galactopyranosyl-(1.4)--D-glucopryanose 15. Identify the correct structural features of purines and pyrimidines. a. purines contain one heterocyclic ring and pyrimidines contain two heterocyclic rings b. purines contain only three ring nitrogens and pyrimidines contain only two ring mitrogens c. purines contain two heterocyclic rings with only three ring nitrogens d. purines contain two heterocyclic rings and pyrimidines contain one heterocyclic ring e. pyrimidines contain one heterocyclic rings with only three ring nitrogens. A bacterial plasmid is shown in the figure below. Answer the following questions (16-17) related to this plasmid 16. When this plasmid is present in a bacterial cell it makes it. a. Resistant to ampicillin and tetracycline. b. Resistant to ampicillin but not tetracycline. c. Sensitive to ampicillin and tetracycline. d. Sensitive to ampicillin and resistant to tetracycline. e. Resistant to ori. 17. Pstl, EcoRI, Hindill and BamHI are all examples of a. DNA polymerases. b. DNA Ligases c. Type Il restriction endonucleases d. Type 1 and type Ill restriction endonucleases. e Nucleotide kinases.

Answer 1

12) Phosphorylation adds a phosphate group to proteins Dephosphorylation involve removal of a phosphate group from a protein. Protein kinase catalyses Kansfer of phosphate from ATP to its protein substrate while protein phosphatase catalyses the transfer of the phosphate from a phosphoprotein to a water melecule ATP ADP Phosphorylated protein Dephosphorylated Protein ADP C ATP. Phosphorylation is caused by kinase involves Conversion of ADP to ATP Dephosphorylation caused by phosphotose involves convusion of ATP to ADP. Answer: (c)