biochem quest. Each of the following reagents or conditions will denature a protein. For each, describe what the re...
Show the melting curve of a protein and label the Tm and indicate whether the protein is found in its native structure or in a denatured state.
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...