Question

LAB 6B PRELAB QUESTIONS To be turned in at the beginning of each lab period. You will not be allowed to proceed without completing this section. Name: O-chem prela Day/Time: 1. Why is it important to have a uniform silica layer (even top and no bubbles) in column chromatography? 2. What is the purpose of using ethanol when mashing spinach leaves before extraction with dichloromethane (DCM)? 3. What percent of B-carotene can you expect spinach leaves to contain? Cite your source.

Answer 1

1 Column chromatography is a method of separation of components of a mixture and thereby purifying them. It is based on the polarity differentes of molecules. It is very important to hack column properly and ensure there is no bubble present so as to achieve proper separation of components. If there will be no uniformity Some part of one component will come first and rest will come with other Components thereby creating foroblem is separation