Question

OH HAN OH IZ (CH2), CH 9 HEN 6

Examine the polypeptide in its major ionization state at pH 1.

-How many amino acids are present in this peptide?

**Can you please circle them and label? I'm having a hard time identifying where they are. The chart I have seems off from what I see here.

-Also, at what pH in an isoelectric focusing gel should you look for this purified peptide? Explain.

Thanks for the help!

Answer 1

माया serine Cystein ser Valine HN Arginine In above peptide there four amino add present, which are as below I HH - HH₂ a CH₂ - CH₂ - CH - H - 6 = H₂ Arginine H HH- & - cooH custeine erhush Зн serine &- cooH CH-OH 19) Hud- & - COOH valine CH

Iso electric focusing is one of the commonly method used for separation of proteins During the separation process, the molecule in the sample accumulate in specific and predictable locations in the medium, regardless of their initial distribution This technique is applicable mainly to the fractionation of amphoteric molecules such as peptides that can acts as both acids and bases. Sodium dodecyl sulfate polyacrylamide gel electrophoresis provides as second dimen- sion of separation of proteins. Very subtle differences in the pl values of proteins differing in pl by less than ool pH unit are resolved by Iso electric gel focusing and separation of proteins with p as low as n.ool pH unit,