Question

Name Date Lab Section I was present and performed this exercise DATA SHEET 11-1 Differential Blood Cell Count OBSERVATIONS AND INTERPRETATIONS Record your data from the differential blood cell count in the table below. As you count the 100 white blood cells, make tally marks in the appropriate boxes. Then calculate the percentages of each type and compare them to the expected values Normal Blood Segmented Band Lymphocytes Neutrophils Neutrophils Monocytes Eosinophils Basophils Number Percentage 25-33% Expected percentage 55-65% <0.2% 2 available, count 100 white blood cells as in step 1 above with an abnoma blood smear. Abnormal Blood (Condition: Segmented Band Monocytes Lymphocytes Neutrophils Neutrophils Eosinophils Basophils Number Percentage 3-7% 25-33% 55-65% Expected percentage SECTION 11 Hematology and Serology 749

Answer 1

I PEA IMSA name e Polyethyl alcohol agar election Mannitol salt a qar Purpose Isolation of pathogenic staphylocorit s Enaymane digest of! Casein, animals boefentract, o-mannitoli Isolation of grampos it the Streptococa, staphylococel Casein enaymahe hadrolysate, soyas na Carbon I pephone Endymanic digest of animal casein, beet extract Allroogen carcin enaqnyahe hydrolysate, soya pephone! I undradeforand undefined aman doh nad undefined Defined undefined Proteose pephone to g/h, Him peptone B # -1912, Nael -489/2, D-mannitol.. 10912, Phenol red-o. Dargle Agar 189/L, PH cat arc) 9. 10.2 Gaselu e na yule hydrolysates , soy a pephone-igle, sodium Chloride Sg/L, PEA - 2.5 g2, Agar-i8gl6 PH Catarc) - 4.3 I 0.2 Belective ingredients Differential ingredient A high salt coucoutation of 4.8% Nael inhibees growth of most bacteria since D. mangirl change colour of aqur medium making it difficult to defferentiate speues (if appleable) T Medium Selective selective Madium type

ВА Maec Mac Conkey agar. Еме Ethylenomethylene bluo agar Icolanon and dipperenniakon of gram negative enteric bacte. Isolanion Escherichia coli from pharmaceutical products Blood agar plate Isolation and cut hva hoy of fashidoue mepcrobas litce Neisseria, streptococci Hm pephone Bitruphose provides carbon monogen, amino acids, Vitamins im peptone B # -logh, Tryphoce - log/t, wael-sgle, lactose monokaduate - Carbon source Panereable dipost of gelation, pephones emeat, casein) - vitrogen source. Pephe dig ost of animal source of carbon, nitrogen, lactose, sucrose - fermontable carbohydrate Peptic digest of animal tissue Hogh, Digotassiumphosphate - Igle, lactose, sucrose rgh, Eosin-y-ouglL, Methylphe blue Fo.o65 g/L, Aglar -13.89(LI foobs PH -7.21 0.2 102 not applicable for the Differenbal medium for Cuot PHA applicable) Pephoner (meat & casein 3gh, Pancreatic digest of golahn- (4 g/L, lactose-10q, Bile salte- Irgl Mach-sg/h, crystalviolet - ooolgll Nedtral red - 0.03911, Agar 13:5gle PH - 9.110.2. Crystal violet and bile calts prenent growth of grampositore organisms allowing isolation of gram nepative One which is differential medium. selective medium, undefined seloctive medium, unde for medium Neutritious medium, undefo-

HE Hertoon enteric agar medium Isolation of salmonella a chigella speres from enterte pathological spoumons. Proteose pophone alt as carbon, mihogen aminowed Source undefined medium Protease pephone- lagle, yeast .3ge, lackse-legle, sucrose ogle Salien I gle, Bile salts mixhine - qgle, Nael-sgle, sodium this sulfate - sgle, terric ammonium litrate alt gle, and Fuschen o.1916, BrBoobs gle, Agar lugl, PH (28) - 7.5ton, or has lectore iwcrose, salon, pephone iph indicators, has ahore & Puhibeoors of age gram the org. Becoz. salmonello, uso Shigella popwone instead produrnar of sugar instead of sugar producing blue-green color, normal gut batterpa "able to use sugar produce yellow orange elettve, dreperental agar medium where of

XID Xylose-dyeine deoxy cholate agar Isolation of salmonella Raphi & other Salmonella speurs undefined medium Jackoso i sucrose yoast extract -Carbohydrate source. - Nitrogen source yeast extract 39/1, L-lysine-igle, dachose tagge, Sucrose - Aigle, Xylose -2.6 g/L sodium chloride -259/2 Sodium deoxy cholate - 2.8916 Phenolred-0.8gle sodium thio sulphate - 6.89/h, Ferric ammonium citrate 0.8 gl Agar-ingle, pH ea FC) - 4.4+0*2 selective medium and differential medium (unea for accovery of salmonella & Shigella) w

1 Eosino BON Normal blood Symphocytes (Neutrophils Se queured Band neutrophil monocytes 26 2 "56 Nuwber an 4% 2% 1-3% Percentage 9 4 56% 5-br/ % 3-74 25-334 Expected percentage Total WBC -14,000/mm3 Abnormal blood CondiBani Oruary tract infection. 4% 290% Number 12 34 1 percentage 34 %. 29 y 72% 1-3%. 2004 25.337. 3.74. I 55-65% Expected%