Question

. A student is going to count her cells using a Coulter counter, but observes that cell confluency is low. Thus, she decides to mix 200 ul (twice as much as usual) of the trypsinized cells with 9.8 mls of PBS before counting. She reads 1230, 1300 and 1340 cells/ml on the coulter counter. What is the concentration of the original harvested cells? (1 point)
please show ALL WORK. circle correct solution, thank you

Answer 1

Trysinized celle have volume of twice of originalf here tryprinized 6 cells have been taken 200m. So, Original cell's volume is &x 200 ML = 100 ML have, when 200 mil trypsinized cells are mixed with 9.8 man in j. l. 98 00 Ml & then total volume of solution - (98 00 + 200 mt. = 10000 mm here dilution factor (DF) of original harvested all = 100g om -Totx Average call on - 100 the counter - 1230 + 1300 +13407 Collosen = 1290 cells surt concentration of original harvested allo - (concentration of all, Ix DF (in dilleted sample) = 1290 cell x 100 - 129000 celle/me M