The following base sequence is found for a mRNA fragment from wild-type E. coli: 5'- UAUCAGUAGAUAAUGUAACC-3'...
The following base sequence is found for a mRNA fragment from wild-type E. coli: 5'- UAUCAGUAGAUAAUGUAACC-3' Assuming that this sequence corresponds to the middle of a gene's coding region, give the amino acid sequence of the protein you would expect the fragment to encode.
Homework Answers
Since this is the middle portion of a coding region of genes.
That's why based on the triplet of codon, there are different amino acid sequence can be found. Whether there are from the starting nucleotide or from second nucleotide or third nucleotide, there are different amino acid are found.
There are three sequence sequence found from these mRNA sequence:
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The following base sequence is found for a mRNA fragment from
wild-type E. coli: 5'- UAUCAGUAGAUAAUGUAACC-3'...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? What is the amino acid sequence of the proteins? Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not? If the T underlined in the above sequence was to go...
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’ (a) Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? (b) What is the amino acid sequence of the proteins. (c) Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not. (d) If the T underlined in the above sequence was to...
7-41 Which of the following pairs of codons might you expect to be read by the...
7-41 Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble? (a) (b) CUU and UUU GAU and GAA CAC and CAU (d) AAU and AGU Below is a segment of RNA from the middle of an mRNA 7-42 5'-UAGUCUAGGCACUGA-3 ' If you were told that this segment of RNA was part of the coding region of an mRNA for large protein, give the amino acid sequence for...
can i get help with this question please Reference Sequence Wild-Type DNA Template Sequence: mRNA Sequence:...
can i get help with this question please
Reference Sequence Wild-Type DNA Template Sequence: mRNA Sequence: Amino Acid Sequence: TAC ACC TTG GCG ACG ACT AUG UGG AAC CGC UGC UGA Met-Top-Asn--Ars--Y-STOP NS. Mutated DNA Template Sequence #5: TAC ACC TTG GGA CGA CT Compare the mutated DNA#5 with the wild-type DNA sequence. Do you observe a substitution, deletion, or insertion mutation? The mRNA sequence derived from mutated DNA #5 is AUG UGG AAC CCU GCU GA Use Table 10.3...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence,...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
4. Given the following information: One strand of a section of DNA isolated from E. coli...
4. Given the following information: One strand of a section of DNA isolated from E. coli reads 5’-GTAGCCTACCCATAGG-3’ 3’ CATCGGATGGGTACC’5 Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be (please include orientation)? How many different polypeptides chains could potentially be made from this sequence of RNA? Would the same polypeptide chains be made if the other strand of the DNA...
1. You are studying a mRNA found in E. coli that appears to encode two separate...
1. You are studying a mRNA found in E. coli that appears to encode two separate polypeptides (there are two separate starting points of translation). You would like to make these same proteins by putting this mRNA into a human cell. Assuming you have the ability to make any RNA molecule with a particular sequence, what RNA(s) would you synthesize to be able to express the two polypeptides in a human cell. Describe what you would need to add or...
Below are several DNA sequences that are mutated compared with the wild-type sequence: 3-TAC TGACTGACGAT C-5....
Below are several DNA sequences that are mutated compared with the wild-type sequence: 3-TAC TGACTGACGAT C-5. Envision that each is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the template strand. Construct the complementary DNA sequences (indicating 5' and 3' ends) for each mutated DNA sequence, then transcribe (indicating 5' and 3' ends) the template strands, and translate the mRNA molecules using the genetic code, recording the resulting amino acid...
The following mRNA sequence is taken from the middle of exon 3 in a mature mRNA...
The following mRNA sequence is taken from the middle of
exon 3 in a mature mRNA that has many exons, and the mRNA encode 9
amino acids.
Question: Knowing that this mRNA does not have an early
stop codon, which of the reading frames shown is the correct one
for this mRNA? Write down 1, 2, or 3 as your answer. Explain
why?
5'..AGUGAUUCGAUACAGCUAGCGGACAGCUA...3 1 ... Reading frames: Z E hahaha ...hohohoh
The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in...
The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in the following table differs from wildtype by a single point mutation. Using this information, determine the mRNA sequence coding for the wild-type polypeptide. If there is more than one possible nucleotide, all list possibilities. Mutant Amino Acid Sequence of Polypeptide Trp-Trp-Trp Met Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met Trp-Trp-Trp-Met-Arg-Lys-Trp-Thr-Met Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met
The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in the following table differs from wildtype by a single...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? What is the amino acid sequence of the proteins? Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not? If the T underlined in the above sequence was to go...
7-41 Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble? (a) (b) CUU and UUU GAU and GAA CAC and CAU (d) AAU and AGU Below is a segment of RNA from the middle of an mRNA 7-42 5'-UAGUCUAGGCACUGA-3 ' If you were told that this segment of RNA was part of the coding region of an mRNA for large protein, give the amino acid sequence for...
can i get help with this question please
Reference Sequence Wild-Type DNA Template Sequence: mRNA Sequence: Amino Acid Sequence: TAC ACC TTG GCG ACG ACT AUG UGG AAC CGC UGC UGA Met-Top-Asn--Ars--Y-STOP NS. Mutated DNA Template Sequence #5: TAC ACC TTG GGA CGA CT Compare the mutated DNA#5 with the wild-type DNA sequence. Do you observe a substitution, deletion, or insertion mutation? The mRNA sequence derived from mutated DNA #5 is AUG UGG AAC CCU GCU GA Use Table 10.3...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
1. You are studying a mRNA found in E. coli that appears to encode two separate polypeptides (there are two separate starting points of translation). You would like to make these same proteins by putting this mRNA into a human cell. Assuming you have the ability to make any RNA molecule with a particular sequence, what RNA(s) would you synthesize to be able to express the two polypeptides in a human cell. Describe what you would need to add or...
Below are several DNA sequences that are mutated compared with the wild-type sequence: 3-TAC TGACTGACGAT C-5. Envision that each is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the template strand. Construct the complementary DNA sequences (indicating 5' and 3' ends) for each mutated DNA sequence, then transcribe (indicating 5' and 3' ends) the template strands, and translate the mRNA molecules using the genetic code, recording the resulting amino acid...
The following mRNA sequence is taken from the middle of
exon 3 in a mature mRNA that has many exons, and the mRNA encode 9
amino acids.
Question: Knowing that this mRNA does not have an early
stop codon, which of the reading frames shown is the correct one
for this mRNA? Write down 1, 2, or 3 as your answer. Explain
why?
5'..AGUGAUUCGAUACAGCUAGCGGACAGCUA...3 1 ... Reading frames: Z E hahaha ...hohohoh
The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in the following table differs from wildtype by a single point mutation. Using this information, determine the mRNA sequence coding for the wild-type polypeptide. If there is more than one possible nucleotide, all list possibilities. Mutant Amino Acid Sequence of Polypeptide Trp-Trp-Trp Met Trp-Trp-Trp-Met-Arg-Asp-Trp-Thr-Met Trp-Trp-Trp-Met-Arg-Lys-Trp-Thr-Met Trp-Trp-Trp-Met-Arg-Glu-Trp-Met-Met
The wild-type sequence of part of a protein is Trp-Trp-Trp-Met-Arg-Glu-Trp-Thr-Met Each mutant in the following table differs from wildtype by a single...
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