Question

Below are several DNA sequences that are mutated compared with the wild-type sequence: 3-TAC TGACTGACGAT C-5. Envision that each is a section of a DNA molecule that has separated in preparation for transcription, so you are only seeing the template strand. Construct the complementary DNA sequences (indicating 5' and 3' ends) for each mutated DNA sequence, then transcribe (indicating 5' and 3' ends) the template strands, and translate the mRNA molecules using the genetic code, recording the resulting amino acid sequence (indicating the N and C termini). What type of mutation is each? Mutated DNA Template Strand #1: 3'-TACTGTCTGACGAT C-5' Complementary DNA sequence: mRNA sequence transcribed from template: Amino acid sequence of peptide: Type of mutation: Mutated DNA Template Strand #2: 3-TACGGAСTGACGATC-5' Complementary DNA sequence: mRNA sequence transcribed from template: Amino acid sequence of peptide: Type of mutation: Mutated DNA Template Strand #3:3-TACTGACTGACTAT C-5' Complementary DNA sequence: mRNA sequence transcribed from template: Amino acid sequence of peptide: Type of mutation: Mutated DNA Template Strand #4:3-TACGACTGACTAT C-5' Complementary DNA sequence: mRNA sequence transcribed from template: Amino acid sequence of peptide: Type of mutation:

Answer 1

» Wild type Sequence template DNA Strand 2 TACTO 3'+ TAC TGACTG ACG ATC-5' 5'- AUG ACUGACUGCUAG-3' [mRNA) Met The Asp eys Stop codon. wild type Polypepticle? N termini - Met-The-Asp-Cys-C termini 2 > Stranded → Mutated DNA Template 3'-TACTGICTG ACGATC-5' [ template DNA strand] 5'- A UGACAGACUGCUAG -3? [mRNA ] Cys stop codon Met The Asp Polypeptide > N termini - Met - Tho - Asp-Cys - C termins Type of Mutation - Silent Mutation ** sident mutation is a change in the nucleotide Sequence of a gene that does not alter the Amino acid sequence of the encoded protein. CS$canned with CamScanner

Mutated DNA template strand a 3'-TAC G GACT GACGATC-5' (DNA template] 5-A UG CCU GACUG CUA G-3'[mRNA] Met Pro Asp cys Stop codon Polypeptide & N termini - Met-Pro-Asp-Cys- C termini Type of Mutation Missense Mutation ** Missense mutation as when change of base Causes the Substitution of a different amino Poly acid. > Mutated DNA template strand 37 3'-TAC TGACTG ACTATC-5' [ DNA template] 5²-A UGACUGACUGAVAG-3' [mRNA Met Thir Asp Stop Codon termini Polypeptides N termini - Met-Tho - Asp-C Type of Mutations Nonsense mutation ** Nonsense mutation introduced in the Stop Colon Present csscanned with CamScanner occur when a DNA Sequence in wild type- Stop Codon t's before the

. Mutated DNA template strand u 3-TAC GACTGACTATC-5' [DNA template] 5-A UG CUGACUGAUAG-3? [mRNA? Met Leu The Asp Polypeptides N termini - Met - Leu - Tho - Asp - c termini Ty be of Mutation Frameshift mutation [deletion] ** H is a genetic mutation caused by deletion or insestion in a DNA sequence that shifts the way the sequence is read during translation. CScanned with CamScanner