Question

Answer The following Please,

Name: Date: Transcription/Translation Practice Worksheet 1. Use the following DNA strand: TAC GTC ACG AGA TGA GTT ATC ATT A. What is the mRNA synthesized from the DNA strand? B. What is the amino acid sequence that is then translated from this mRNA strand? 2. Use the following DNA stand: TAC TTG GCC ACG GAC TAA CAT GCA A. What is the complementary DNA strand of the above DNA strand? B. Using the complementary DNA strand, what is the mRNA strand? C. Take the mRNA and translate it into the amino acid sequence.

Answer 1

Question

DNA strand: TAC GTC ACG AGA TGA GTT ATC ATT

1.

A. The mRNA will be complimentary to DNA sequence. The T will be replaced with A, G with C , C with G and A with U.

mRNA: AUG CAG UGC UCU ACU CAA UAG UAA

B. The amino-acid sequence will be following:

Methionine-glutamine-cysteine-serine-threonine-glutamine-stop codon

2.

DNA strand: TAC TTG GCC ACG GAC TAA CAT GCA

A. The complimentary DNA strand is

ATG AAC CGG TGC CTG ATT GTA GCT

B. The mRNA strand will be like

AUG AAC CGG UCG CUG AUU GUA GCU

C. The amino acid sequence will be

Methionine- asparagine-arginine-serine-leucine-isoleucine-valine-alanine

3.

Normal DNA strand:

CGA TTG GCC ACG GAC TAA CAT

Mutated DNA sequence: CGA TTG GCC CCG GAC TAA CAT

A. mRNA from original DNA strand:

GCU AAC CGG GGC CUG AUU GUA

B. This is a silent mutation.

C. The fourth amino-acid remain glycine.

4.

Normal DNA sequence: TAC TTG GCC ACG GAC TAA CAT

Mutated DNA sequence: TAC TTG GCC CGG ACT AAC AT

A. mRNA of wild type DNA sequence is:

AUG AAC CGG UGC CUG AUU GUA

B. This is mis-sense mutation

C. The last amino acid sequence get changed due to deletion of last amino acid.