Question

Fill in the complementary base pairs for the strand below STACGCCCATTI TAGA ATTGCGTGGGCATTAAGTTTTA TACC3 DNA sequences 3' I Find and put a square around the TATA box (promoter) in the double stranded DNA above Find and circle the terminator sequence (GGGCG) in one of the strands above The strand with promoter and terminator is the strand of interest: using the template strand, synthesize an mRNA sequence mRNA molecule in the boxes below; pay attention to polarity 53: Find and circle the start codon (AUG) in the mRNA strand above mRNA codons Starting with the start codon, set your reading frame by copying the resulting codons into the boxes below Using a codon decoder table, write the amino acids translated by the codons above into the boxes below The polypeptide ends when a stop codon is reached peptide chain

Answer 1

DNA SEQUENCE

5'

T

A

C

G

C

C

C

A

T

T

T

T

A

G

A

A

T

T

G

C

G

T

G

G

G

C

A

T

T

A

A

G

T

T

T

T

A

T

A

C

C

3'

3'

A

T

G

C

G

G

G

T

A

A

A

A

T

C

T

T

A

A

C

G

C

A

C

C

C

G

T

A

A

T

T

C

A

A

A

A

T

A

T

G

G

5'

since the 3' $\rightarrow$ 5' strand contains both PROMOTER ( Blue colour) and TERMINATOR (Orange colour) it is referred as TEMPLATE STRAND

mRNA SEQUENCE

5' AUGCGGGUAAAAUCUUAACGCACCCUAA 3'

mRNA codons :

AUG- Methionine CGG-Arginine GUA- Valine AAA- Lysine UCU- Serine UAA-stop