Question

how to solve 9-10

9. After plating 0.1ml of a 10 dilution, 158 colonies grow. What is the OCD? (CFU/ml) 158 3,514 (105) = 1.58×10 CFU/mL a account for Sci not. dec. 10. After plating 0.2 ml of a 10 tube dilution, 437 colonies grow. What is the CFU/ml or how would you report this info? 437 0/2 OCD 2.16x100 CP /ML 0.2110-5)

Answer 1

Ansi-9) Given Amaunt Plated = o.lm! Dilution factor los colonies - 158 Solution: - No. of Colonies in a late count x dilution factor = 158 1o original tube - 158 x 106 OCD = 1.58 X10

Ansi-10) Given Amaust Plated :-o amb Dilution factor los colonies :- 437 solution - No. of Colonies in - Plate Caunt y original tube - dilution Z 437 0.2 = 218.5 X10 I OCD = 2.185*108 factor x lou- 0.2 coulme cru- mearures viable cells In above solution 2.185x108 viable sol cells are present in Iml af giren higuid.