1. What is the minimum volume to use in the Spec 20 cuvette in order to...
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What is the minimum volume to use in the Spec 20 cuvette in order
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1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
my questions are the following: A) what is the concentration of the stock solution of LDH?...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
1) What pipetman would you use to drspen 600ul? 17ul? 70ul? 2) What size pipet (1,...
1) What pipetman would you use to drspen 600ul? 17ul? 70ul? 2) What size pipet (1, 5, 10, 25ml) would you use to dispense: 2ml? 8ml? 20ml? 3) Convert: a. 5 x 10 ml to ul b. 7 ml to ul c. 50 g/1 to mg/1 d. 0.4 mg/1 to mg/ml e. 75 mg/ml to ug/ul f. 10 M to mM g. 10 M to uM 4) Divide 2.5 x 107 by 103 5) Multiply 5.2 x 107 by 103...
7. The Absorption Spectrum of Cobalt(II) Chloride Procedure Getting Started 1. Your laboratory instructor may ask...
7. The Absorption Spectrum of Cobalt(II) Chloride Procedure Getting Started 1. Your laboratory instructor may ask that you work in groups rather than alone. 2. Obtain directions from your laboratory instructor for discarding the solutions that you will use in'! this experiment. 3. Obtain instructions for using your spectrophotometer. 4. Obtain your unknown. Making the Measurements 1. Mark each of 7 dry 18 x 150-mm test tubes with one of a series of identification numbers running from 1 to 7....
1. What volume of 17.4 M HCl should be used to prepare 500 mL of a...
1. What volume of 17.4 M HCl should be used to prepare 500 mL of a 6.00 M solution of HCl? A. 1450 mL B. 209 mL C. 0.209 mL D. 172 mL E. 2.9 mL 2. A student removes 25 mL of 3.0 M NaOH and dilutes it to a final volume of 350 mL. What is the concentration of the resulting solution.? A. 42 M B. 0.024 M C. 75 M D. 0.10 M E. 0.21 M 3. How many moles of Na2SO4are required to make a solution...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
Pre-Lab Assignment 1) Draw the reaction mechanism for the reaction. 2) Determine the limiting reagent. Procedure...
Pre-Lab Assignment
1) Draw the reaction mechanism for the reaction.
2) Determine the limiting reagent.
Procedure 1 Place approximately 1 g of stilbene dibromide prepared in last week's experiment in a 50 mL round bottom flask. Record the exact mass. Add 0.8 g of KOH and 4 mL of triethylene glycol from the syringe provided in the fume hood (rinse any crystals from the sides of the flask while adding triethylene glycol). 2 Heat the stirred reaction mixture to a...
my questions are the following:
A) what is the concentration of the stock solution of LDH?
B) Based upon Part B of the protocol, show your calculations to
make the 250ug/ml LDH solution.
C) Based upon Part B of the protocol, show your calculations to
make the 25ug/ml LDH solution.
You will be given 60 ul of LDH stock solution. You need to determine the protein concentration of this stock solution and then perform a serial dilution to end up...
1) What pipetman would you use to drspen 600ul? 17ul? 70ul? 2) What size pipet (1, 5, 10, 25ml) would you use to dispense: 2ml? 8ml? 20ml? 3) Convert: a. 5 x 10 ml to ul b. 7 ml to ul c. 50 g/1 to mg/1 d. 0.4 mg/1 to mg/ml e. 75 mg/ml to ug/ul f. 10 M to mM g. 10 M to uM 4) Divide 2.5 x 107 by 103 5) Multiply 5.2 x 107 by 103...
7. The Absorption Spectrum of Cobalt(II) Chloride Procedure Getting Started 1. Your laboratory instructor may ask that you work in groups rather than alone. 2. Obtain directions from your laboratory instructor for discarding the solutions that you will use in'! this experiment. 3. Obtain instructions for using your spectrophotometer. 4. Obtain your unknown. Making the Measurements 1. Mark each of 7 dry 18 x 150-mm test tubes with one of a series of identification numbers running from 1 to 7....
Pre-Lab Assignment
1) Draw the reaction mechanism for the reaction.
2) Determine the limiting reagent.
Procedure 1 Place approximately 1 g of stilbene dibromide prepared in last week's experiment in a 50 mL round bottom flask. Record the exact mass. Add 0.8 g of KOH and 4 mL of triethylene glycol from the syringe provided in the fume hood (rinse any crystals from the sides of the flask while adding triethylene glycol). 2 Heat the stirred reaction mixture to a...
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