Question

1. You want to prepare a 1:50 dilution of your protein extract in a total volume...

1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water.

2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA?

3.

Which of the following statements about enzymes is true?

Select one:

a. Enzymes increase the rate of a chemical reaction by raising the activation energy for the reaction

b. Enzymes react with their substrate (form chemical bonds), forming an enzyme-substrate complex, which irreversibly changes the enzyme

c. Enzymes work equally well at any temperature

d. Enzymes can typically bind many types of substrates

e. None of the above

4. A Bradford assay is one application of using a “standard curve.” One uses known concentrations of BSA (and their measured absorbance at 595 nm values) to calculate unknown concentrations of some solution of interest (i.e. our protein extract). For example, you measure the absorbance readings of known concentrations of BSA and make a “y vs. x” plot: Absorbance at 595 nm vs. Concentration (ug/mL). You obtain a regression equation of y = 0.096x + 0.0286. Suppose the Abs595 reading of a 1:10 dilution of a protein extract was 0.26. What is the protein concentration of the UNDILUTED protein extract?

Please answer all

0 0
Add a comment Improve this question Transcribed image text
Answer #1

QUESTION 1 In order to determinate how much protein you need to make a dilution 1:50 of 1000 ul, divide 1000 ul by 50 parts Y

Add a comment
Know the answer?
Add Answer to:
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions
  • 1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...

    1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...

  • Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...

    Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...

  • I forgot to put down that the observance (=OD) at 280nm is 0.679A Experiment 8: Bradford...

    I forgot to put down that the observance (=OD) at 280nm is 0.679A Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...

  • Could you please help me figure this out. This is based on the 'protein concentration quantification'...

    Could you please help me figure this out. This is based on the 'protein concentration quantification' lab we conducted in class. We did both the Lowry and Bradford Reagent methods. There were 3 unknown protein concentrations (unknown A = Lactase; unknown B= Insulin; unknown C= BSA). We used 0.5 ml of each protein sample for our unknowns. Below are my unknown concentrations for the Lowry method: Unknown A = 225.3 ug/ml Unknown B = 238.1 ug/ml Unknown C= 517.5 ug/ml...

  • Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....

    Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...

  • You have 0.5 mL sample of protein extract (absorbance 0.5930) with a concentration of 181.2 ug/mL....

    You have 0.5 mL sample of protein extract (absorbance 0.5930) with a concentration of 181.2 ug/mL. How many ug (micrograms) of sample do you have?

  • 1.How would you make 1 mL of a 1 to 25 dilution of yeast crude extract?...

    1.How would you make 1 mL of a 1 to 25 dilution of yeast crude extract? 2. Enzyme activity measures: A)The number of polypeptide molecules B)The number of substrate molecule C)The energy used in the reaction D)The rate at which substrate is processed by the enzyme

  • If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water...

    If you are making a 1:10 dilution of a 10mg/ml BSA solution using 90ul of water as diluent, how much 10mg/ml of BSA would you add? What would be your final concentration of BSA? If you assayed both these solutions using the Bradford assay which one would have a higher O.D.?

  • In lab, you diluted your unknown protein solution by combining 14 ?L of unknown protein solution...

    In lab, you diluted your unknown protein solution by combining 14 ?L of unknown protein solution with enough PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten minutes, the absorbance at 595 nm was 0.206. Using the standard curve from the previous question, calculate the total concentration of protein (in ?gmL) in the original sample. Slope = 0.02371    Intercept = 0.01132    r2 = 0.973

  • Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how...

    Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT