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1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
I forgot to put down that the observance (=OD) at 280nm is 0.679A Experiment 8: Bradford...
I forgot to put down that the observance (=OD) at 280nm is
0.679A
Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
You have obtained 150 µL of a protein sample of which you want to perform a...
You have obtained 150 µL of a protein sample of which you want to perform a Bradford Assay. You perform a 10-fold dilution of your sample and use 10 µl of this dilution to quantify the amount of protein using a Bradford assay. You obtain an absorbance value of 0.63. Using the standard curve and equation you obtained in class, determine the concentration of protein (µg/µL) of your original sample. Show your work. equation: y=0.0139x-0.0153
4) You have been given the job of designing an experiment to test the effects of...
4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50L of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 595mm of 0.6,...
BSA Standard (mg/mL) BSA Working Solution (0.5 mg/mL) ddH2O (µL) BSA Standard (µL) 1 x Bradford...
BSA Standard (mg/mL) BSA Working Solution (0.5 mg/mL) ddH2O (µL) BSA Standard (µL) 1 x Bradford Reagent (µL) OD595nm Reading 0.00 0 500 25 975 0.00 0.025 25 475 25 975 0.43 0.05 50 450 25 975 0.45 0.1 100 400 25 975 0.469 0.15 150 350 25 975 0.571 0.2 200 300 25 975 0.621 0.3 300 200 25 975 0.638 0.4 400 100 25 975 0.713 1. Construct a standard curve and plot. 2. Your unknown sample yields...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that...
Quantification of Total Protein Using Bradford Protein Assay Pre-Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
I forgot to put down that the observance (=OD) at 280nm is
0.679A
Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...
4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50L of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 595mm of 0.6,...
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