Question

4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50L of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 595mm of 0.6, what is the approximate concentration of polymerase in the stock solution? Use this standard curve to get to your answer. 1.2 ооооо ADTADA DITA Absorbanc 595 nm TAD ATOARD ATOSTADOS PADJADTOD OTTATA ADTAZADA DATDA 0 0.25 1.75 2 2.25 0.5 0.75 1 1.25 1.5 BSA standard (mg/ml) 09039ADTA DETRO DODADADADT

Answer 1

The approximate concentration of the carona polymerase stock will be as follows

The OD 0.6 at an obsorbance of 595nm on the Y axis corresponds to 1mg on the X-axis of BSA std.

4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50uL of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 59mm of 0.6, what is the approximate concentration of polymerase in the stock solution? Use this standard curve to get to your answer. Absorbanc 595 nm ADJAD CADET 0 0.25 0.5 1.75 2 2.25 0.75 1 1.25 1.5 BSA standard (mg/ml)

Therefore it is assumed that the sample which was studied in the Bradford assay has 1mg/ml of protein. But however an initial dilution was done

where a dilution nof the polymerase sample is done: 50ul with 100ul of water which is has a dilution factor of 3 ((50+100)/50)

so multiply the concentration of the sample which was used to plot in the graph with the dilution factor

1 X 3 = 3mg

Therefore the concentration of the Carona polymerase stock is 3mg/ml