Ans:- The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. In order to find a standard curve , one must use varying concentration of BSA in order to create a standard curve with concentration plotted on the x-axis and absorbance plotted on the y-axis. This curve is used to determine the concentration of the unknown protein.
From the graph we can obtain the equation of the line y=mx+b ,with R2 value as close to 1 as possible. R represents the sum of the square values of the fit substracted from each data point .
Here y= absorbance
m=slope
x= concentration
In the given question we are to use the line equation
y=0.350
b=0.0097
given equation y=0.0004x +0.0097
0.350=0.0004x+0.0097
x= 0.350-0.0097/0.0004
=850.75 ug/ml
the concentration at an absorbance of 0.35 is 850.75ug/ml
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
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