Question

Weight Using SDS-PAGE Quantification of Total Protein Using Bradford Protein Assay Pre- Lab Your pre-lab assignment is to plan how you will prepare your BSA standard solutions for the calibration curve. A 2 mg/mL BSA stock solution will be available. You can simply dilute with deionized water. You will need to make 25 microliters of each of the following concentrations. For the first concentration, you will need to make 160 microliters so that the BSA volume is large enough that it can be measured with a micropipettor. 25 micrograms/mL 300 micrograms/mL 600 micrograms/ml 900 micrograms/mL 1200 micrograms/mL Please write up a plan for how you will prepare these standards and submit it here.

Answer 1

Standard is 2 mg/mL = 2000 Mg/mL For 2000 ani ze For 25 Mg/mL, 25x160 I 2000 - 2 ML (Take 2ML of standard i - dilute it up to 160 ML with deionized water) Mg/mL, 25x300 = 3.75 ML/3.75 ML standard & deionized water upto Rs 25 ML) 600 MgimL. 25x600 = 7.50 ML (1 50 ML standard & deionized 2000 water up to 25 ML) 900 Mylne 25x900 = 11:25 ML (11.25 ML standard + deionized water up to 25 ML) 1200 MgimL 25x1200 -= 15 ML (15 ML standard + deionized water up to 25 ML) for 2000 = 11.25 ML Glosa For 2000