Question

Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Sid 1 Std 2 Sid 12 6 3 Sid 4 1.5 concentration (mgml Idilution factor from volume of concentrate volume of diluent final dilution of stock final volume/tube You have a stock solution that contains 2 mg/ml of protein You want to make a set of standards with the following concentrations: 150 [g/ml, 50 [g/ml, 16.67 g/ml, and 5.56 g/ml. You need a minimum of 300 ul of each standard. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Sid 1 Sid 2 Sad 3Std 4 concentration dilution factor / from? volume of concentrate volume of diluent final dilution of stock final volume/tube

Answer 1

tou Ans - cone. (more) so I stalo Std it Stall po - 12 8 3 - 15 dilutions fauts 24 - 2x 24 4x 24 = 8x 24 = 16x volume of cone. se ) - 250 ey 500 12564 500 262514 500 = 31.2509 volume of dinhels (500.550) (500-105) Gro-625) 500 - 31.2524 - 25014 - 375e4 = 437.5 = 4.68.75 Prdlub m = 2 ч, 8, 167 final volume sooly Std I Std I Std Cone. String sougione so egoe 16.07 by lor 5 sogen dilution volume gune. 10 3 75ey 180 = 25e4 1000 28.33 100 islume of dutine (300-75)=22814 (308.25-27844 800-833) - 29.67ey 227.22 final dicting = 13.334 - yox 119.97X 359.77 final volumestuse 300ly Zoo Broll tube = 2.78 -30-278 11€ 300ly

Stock core. darf ceramie 7.65 neemen to prema naci 15. boyal hussa mechhouses & mathuras in s neeshu to melhor do so nos postop ich hores celhus porxh mare moaril most = (osc-ear)) ruar noch har recall 249.988 = (EE.891-aal) NEG. SEIT Parel (xs. t = fasil nechausio to all thes) world m 666 = (1-ass!) neehause sah= (se) osea la meil west 6. = (-aali) maar tota la rullee 10 wood H.586 = (mein l-alld) IN 87.41 = (a nellen ) toca(s (tras) = (a moss-mas-all Hent m 1 10 nachheuges ) soce (1 (or 7 10 % (v/v) و همه tot (8 tad (6) 2521 ( Las carl 0.052/4/2) ( 20 ) = 400 x 0.14 (10) = 1807 0.1X (6grox 125 = 25x 2x10 celesne (106) 50% (20) = 20x 2% (36) = 1.5% (10) = 13.334 11007 2.5 B4 (1988. 97rly (1000) = 1044 (1000-10) = 9904 2 ly licos-2) 99844 (1000) = 40x4 (1000-40) 2960 (1000) = 2014 (100-20) = 980 eu 2 000) =500 (1000.50)=95044 (1000 = 666.6 (1000 offitle) = 335.33 1600 1000 = 7.5 (1000 -78) = 992.5 1000 11) 106 cellslime 7 So 2.X/ 0 sal eiddor wedot 13) 3% hael Chi bsite

Hope it's clear..thanks