Question

You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final amount of standard you will have after all dilutions are completed Sidl Std 2 Std 3 Std 6 Std 4 0.25 Std 5 0.05 20 0.0125 concentration (mg/ml) dilution factor from tube volume of protein Cl) volume of diluent (ul) final volume of standard final dilution TTTF Paragraph Arial . (1200) XIETT E T . . i

Answer 1

1 ho 3 4 5 Conco 2 5 0.05 0.05 0.0125 dien 10020 1085 100/1 10%25 100005 t olas fact H00 = 400 Z000 hrs tubet original 1 I proto 100 125 100 125 too diluent fuu 375 400 315 final Dueto 500 500 500 500 500 8x18 5 5x20 104 400 trytothooo ^ 100x100 toy 416 4x16 = =8XL0 formula > GV = CV (for conc. of protein)

Please rate high.