1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To...
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To do a serial dilution of 10% concentration (not volume) for each dilution, how many uL of liquid do you move between each well? ype your answer as a whole number of uL, but do not type the "uL" label.
2) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. What is the final concentration of well 6 if you transfer 10 uL between each well serially?
3) Suppose you have 6 wells. The first well contains 20 uL of serum. Wells 2-6 contain 40 uL of water. If you want to make 20% serial dilutions, how many uL do you transfer between each well? I mean 20% concentration, not 20% volume. Type your answer as a whole number.
4) suppose you have 6 wells. The first well contains 20 uL of serum. Wells 2-6 contain 40 uL of water. If you want to make 20% serial dilutions, what is the final dilution of well 6, as a fraction? Use the forward slash as a fraction bar with no spaces
Homework Answers
Add Answer to:
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of
water. To...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32,...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
explain question 6 please explain question 8 not 6 Mix 9 mL of water + 1...
explain question 6
please explain question 8 not 6
Mix 9 mL of water + 1 uL of DNA Mix 1 mL of water+9 mL of DNA QUESTION8 You make a 1:50 dilution of yoqr stock, then you make a 1:10 dilution of the 1:50. What is the dilution factor of the final solution? 1:100 ○ 1:500 1:10 1:5 QUESTION9 0.1 mL Click Save and Submit to save and submit.Click Save All Answers to save all answers Save All Answers...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the dilution in tube 1 is added to 4 mL in a second tube. One milliliter from tube 2 is then added to 9 mL diluent in tube 3. What is the substance (plasma) volume in the third tube? 18. What is the specific gravity of a substance if 5 mL of the substance weighs 6.5 g, but the substance is only 50% pure? 19.What...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
explain question 6
please explain question 8 not 6
Mix 9 mL of water + 1 uL of DNA Mix 1 mL of water+9 mL of DNA QUESTION8 You make a 1:50 dilution of yoqr stock, then you make a 1:10 dilution of the 1:50. What is the dilution factor of the final solution? 1:100 ○ 1:500 1:10 1:5 QUESTION9 0.1 mL Click Save and Submit to save and submit.Click Save All Answers to save all answers Save All Answers...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the dilution in tube 1 is added to 4 mL in a second tube. One milliliter from tube 2 is then added to 9 mL diluent in tube 3. What is the substance (plasma) volume in the third tube? 18. What is the specific gravity of a substance if 5 mL of the substance weighs 6.5 g, but the substance is only 50% pure? 19.What...
Most questions answered within 3 hours.
-
20% of all customers subscribe to phone service.
70% of all customers subscribe to internet service....
asked 2 minutes ago -
Write a program to solve the Josephus problem, with the following
modification:
Sample Input:
./a.out n...
asked 2 hours ago -
At the start of a CD it is spinning at a rate of 525 rpm
(revolutions...
asked 3 hours ago -
4. Without doing any calculations, predict whether the observed
∆T would increase, decrease or remain the...
asked 4 hours ago -
Based on the range, which of the following sets of scores has
the greatest variability? 3,...
asked 5 hours ago -
Ripples in a pond travel at a velocity of 3 m/s with one peak
passing a...
asked 5 hours ago -
A man stands on the roof of a building of height 13.0 mm and
throws a...
asked 5 hours ago -
The extent to which assets are financed by borrowed funds and
other liabilities is indicated by:...
asked 6 hours ago -
Explain in detail
Germany is the fifth largest economy
explain what goods and services Germany specializes...
asked 6 hours ago -
The density of platinum is 21.45 g/mL. If a cube of platinum
with a mass of...
asked 7 hours ago -
Accounts Receivable
Sales
A/R Posting
Extended Sales Invoice
Packing Slip
Compare invoice to packing slip 2...
asked 7 hours ago -
Michaella, age 23, is a full-time law student and is claimed by
her parents as a...
asked 7 hours ago