Homework Answers
Ans.) Option B: 1:500
Explanation: For calculating net dilution factor in a series of steps, the dilution at each step in multiplied.
DF = DF1 x DF2
therefore, Net DF = (1/50) x (1/10) = 1/500 or 1:500
Dilution Factor = Vi / Vf
Add Answer to:
explain question 6
please explain question 8 not 6
Mix 9 mL of water + 1...
You transfer 1 mL of stock solution to tube A, add 9 mL of water and...
You transfer 1 mL of stock solution to tube A, add 9 mL of water and mix. You then transfer 1 mL from tube A to tube B, add 9 mL of water and mix. And finally you transfer 1 mL from tube B to tube C, add 9 mL of water and mix. What is the total dilution factor for this series when comparing the stock solution with tube C? (990 is not the answer)
How to solve for question#6? 5 5. Fill in the table below to set up the...
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Molecular Key Calculation - PLEASE NEED HELP TO CALCULATE IT! You are setting up you own...
Molecular Key Calculation - PLEASE NEED
HELP TO CALCULATE IT!
You are setting up you own Master Mix for doing a Not1
restriction digest.
In each digestion tube you will be adding 10 ul DNA and 40 ul of
your Master mix.
You are given 10 x Not1 buffer, 10 ug/ul acetylated BSA, Not1
restiction enzyme 10 units/ul and sterile water.
How much of each of the 4 reagents would you use to make 500 ul of...
please show work so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml...
please show work
so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
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QUESTION 5 An oral solution has a strength of 200 mg/mL. Prepare a 0.4 g dose. TTT 43 (12pt) * T. Arial M Path:p QUESTION 6 Prepare a 0.2 mg dose from a solution labeled 400 mcg/mL. TTT Arial 3 (12pt) : T e so Click Save and Subunit to save and submit. Click Save All Answers to save all answers MacBook Air
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To...
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To do a serial dilution of 10% concentration (not volume) for each dilution, how many uL of liquid do you move between each well? ype your answer as a whole number of uL, but do not type the "uL" label. 2) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. What is the final concentration of well 6 if you...
QUESTION 1 Calculate the volume of 0.5 M KOH solution in mL required to completely neutralize...
QUESTION 1 Calculate the volume of 0.5 M KOH solution in mL required to completely neutralize 25.0 mL of 0.1 M HNO3 solution (a) 25 (b) 5 (c) 10 (d) none of the choices is correct QUESTION 2 20 mL of 0.2 M NaOH solution is required to completely neutralize 20 mL of a standard H2SO4 solution (a) 0.1 M (b) 0.2 M (c) 0.5 M (d) None of the choices is correct Click Save and Submit to save and...
QUESTION 15 A financial manager's financing decisions determine a. both the mix and the type of...
QUESTION 15 A financial manager's financing decisions determine a. both the mix and the type of assets found on the firm's balance sheet b. the most appropriate mix of short-term and long-term financing c. both the mix and the type of assets and liabilities found on the firm's balance sheet the proportion of the firm's earnings to be paid as dividend d. QUESTION 16 Save and Suhmit to save and submit. Click Save All Answers to save all answers. type...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Molecular Key Calculation - PLEASE NEED
HELP TO CALCULATE IT!
You are setting up you own Master Mix for doing a Not1
restriction digest.
In each digestion tube you will be adding 10 ul DNA and 40 ul of
your Master mix.
You are given 10 x Not1 buffer, 10 ug/ul acetylated BSA, Not1
restiction enzyme 10 units/ul and sterile water.
How much of each of the 4 reagents would you use to make 500 ul of...
please show work
so: 1 ml stock 10 ml final -0.5 mL of stock -5 ml final volume While I have used 1:10 as a standard the same rules apply for all dilution factors. Try these problems: x 0.5 X0.5 1. You need to make a 1:25 dilution factor. If you final volume is 25 mL this means that you need_ _mL of stock and mL of solvent to make your final volume of 25 mL. 2. If you needed...
QUESTION 5 An oral solution has a strength of 200 mg/mL. Prepare a 0.4 g dose. TTT 43 (12pt) * T. Arial M Path:p QUESTION 6 Prepare a 0.2 mg dose from a solution labeled 400 mcg/mL. TTT Arial 3 (12pt) : T e so Click Save and Subunit to save and submit. Click Save All Answers to save all answers MacBook Air
QUESTION 1 Calculate the volume of 0.5 M KOH solution in mL required to completely neutralize 25.0 mL of 0.1 M HNO3 solution (a) 25 (b) 5 (c) 10 (d) none of the choices is correct QUESTION 2 20 mL of 0.2 M NaOH solution is required to completely neutralize 20 mL of a standard H2SO4 solution (a) 0.1 M (b) 0.2 M (c) 0.5 M (d) None of the choices is correct Click Save and Submit to save and...
QUESTION 15 A financial manager's financing decisions determine a. both the mix and the type of assets found on the firm's balance sheet b. the most appropriate mix of short-term and long-term financing c. both the mix and the type of assets and liabilities found on the firm's balance sheet the proportion of the firm's earnings to be paid as dividend d. QUESTION 16 Save and Suhmit to save and submit. Click Save All Answers to save all answers. type...
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