Question

Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways that you could dilute a sample to make a 10 dilution? 5. If you count 198 colonies on your 10 tube dilution, how many bacteria/ml were in your sample? 6. You pipette 1 ml of your sample into a 9 ml dilution blank, and then pipette 1 ml of that dilution into a 99 ml dilution blank. Finally you pipette 0.1 ml of that last dilution into a plate. What is the dilution of that plate? MacBook Pro

Answer 1

added to Q ml. Answer for a ten fold (1:10) dilution, Iml of sample 66 diluent. In this case the dilution factor for that test tube will be : ailutin with the help of formula volume of sample volume of samplet factor diluent of 7 ml ImltgmL 0.1 10-1 to 10 since have done senial dilution for three times. so second time dilution factor 10-1 third time dålution factor Note dilution. d. 10-1 factor Total dilution 10+ x 1041 x lot! Answer. Total dil. 2 dilution 10+3 Answer - sim impally dilution factor 1 Itaa loo lo 2 dilution factor in testtube after adding Oil ml to the agar plate, the dilution of the sample on the plate will be

Page Here total dilution in the test tube is on 10-2 100 total is added to the agar plate so when out ml ol the dilution will be dilution XL factor 100 10 11 or lo-3 1ooo dilution factor is reciprocal of total dilution, 8 a to fal dilution = 10+3/ Answer