Question

1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL 0.1mL 0.1mL 0.1mL 3. (A) Calculate the dilutions for each tube. Calculate the dilutions of each plate from transferring 0.1mL from the corresponding tube? Show work in scientific notation. 1 mL 1 mL 1 mL 1 mL 1 mL 9 ml 9 mL 9 mL 9 mL 9 mL Sample 0.1ml 0.1ml 0.1mL 0.1mL 0.1ml TFTC TNTC 1354 345 75 Plaque counts: (B) Which plate count is statistically appropriate to calculate cell density of the original sample known as Original Cell Density (OCD)? Provide a reason for your response. (C) Calculate the OCD (Original Cell Density) of the original sample using the plate from the previous question? Show work below in scientifie notation with correct units. (D) Calculate the CFU or colony count of the last plate? (Hint: OCD-1.0x10' CFU/mL) Use the following diagram to answer the questions below. Show all work 4. (A) Caleulate the dilutions for each tube. Write dilutions in scientific notation. 1.0ml 1.0ml 1.0ml 1.0ml 99ml 99ml 9ml A. B. C. D. 0.1ml 0.1ml 198 CFU 26 CFU (B) Using a countable plate calculate the cell density of the original sample in the flask? Show all work with correct units

Answer 1

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