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Help! Not sure how to do dilutions!
1. Calculate the serial dilutions using the following diagrams....
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL...
Your Name: Solutions and Dilutions 10mL added to 990mL 1mL 5mL added to 5mL 1M HCI...
Your Name: Solutions and Dilutions 10mL added to 990mL 1mL 5mL added to 5mL 1M HCI 1ml added to 99mL 1L Total volume 100mL Total volume 1-10mL Total volume 7(6). Examine the dilution series shown above and calculate the following. Show all work for full credit IDF of A: IDF of B: IDF of C: TDF of A: TDF of B: TDF of C: 8 (1). The stock (starting) solution of HCI was 1M. What will be the concentration of...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial cu...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two...
Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two different approaches you can take (each using three steps/dilutions). Could someone please guide me through the steps of how to solve this dilution problem? I'm at a loss as to where to start
Serial Dilution Questions 1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
1. 2. Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and...
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Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and the volume of water needed to dilute the following solutions: Concentration Volume (in mls) Concentration of Volume (in mls) of Volume (in mls) of diluted of desired available stock stock solution required of solvent solution desired solution (V2) solution (C1) to make the diluted required to (C2) solution (V1) achieve desired volume (V2-V1) 5 mg/ml 100 ml 1000 mg/ml 15% 10 ml 50% 0.250...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 3...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
1. Calculate the serial dilutions using the following diagrams. 1mL 25mL 5mL 5mL 99mL 75mL 45mL 5mL Dilution: 0.1mL .01mL 0.1mL 1.0mL 1.9mL 9.99m 99.9mL 2mL Dilution: - when transferring 0.1mL from the corresponding tube? Show all your work in scientifie notation. Hint: Observe the diagram carefully 2.. Caleulate the dilutions for each tube; then calculate the dilutions for the four plates 1 mL or 1g sample 1 mL 1mL 1mL 1mL 1mL 1mL 1 mL ml 99 mL 0.1mL...
Your Name: Solutions and Dilutions 10mL added to 990mL 1mL 5mL added to 5mL 1M HCI 1ml added to 99mL 1L Total volume 100mL Total volume 1-10mL Total volume 7(6). Examine the dilution series shown above and calculate the following. Show all work for full credit IDF of A: IDF of B: IDF of C: TDF of A: TDF of B: TDF of C: 8 (1). The stock (starting) solution of HCI was 1M. What will be the concentration of...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
1.
2.
Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and the volume of water needed to dilute the following solutions: Concentration Volume (in mls) Concentration of Volume (in mls) of Volume (in mls) of diluted of desired available stock stock solution required of solvent solution desired solution (V2) solution (C1) to make the diluted required to (C2) solution (V1) achieve desired volume (V2-V1) 5 mg/ml 100 ml 1000 mg/ml 15% 10 ml 50% 0.250...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
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