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Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 3...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein....
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
1. 2. Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and...
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Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and the volume of water needed to dilute the following solutions: Concentration Volume (in mls) Concentration of Volume (in mls) of Volume (in mls) of diluted of desired available stock stock solution required of solvent solution desired solution (V2) solution (C1) to make the diluted required to (C2) solution (V1) achieve desired volume (V2-V1) 5 mg/ml 100 ml 1000 mg/ml 15% 10 ml 50% 0.250...
Provided materials 0.01 to 0.05 M stock solutions of red and yellow dyes Dyo [stock] 0.0319...
Provided materials 0.01 to 0.05 M stock solutions of red and yellow dyes Dyo [stock] 0.0319 M V to mix red V of stock 100 ml 1.00 ml 264 mL yellow 0.0196 M 253 mL Experimental Procedure Dilute stock solutions by adding 1.00 mL of stock to sufficient distilled water to provide a total volume of 1.00 L 1.00 ml aliquots of stock solutions of red (0.0319 M) and yellow (0.0196 M) dyes are separately diluted with distilled water to...
To construct a calibration curve we would need to take our stock solution and dilute it...
To construct a calibration curve we would need to take our stock solution and dilute it to different concentrations. We would then test those different concentration in our spectrophotometer to test their absorbance. If we diluted our samples as follows, calculate the resulting concentrations. (HINT: this is dilution which means we can use M1V1=M2V2, where M1 is our original 150 mM concentration, V1 is the volume of the original concentration that we added water to dilute, M2 is the concentration...
Suppose you have 100.00 mL of a solution of a dye and transfer 2.00 mL of...
Suppose you have 100.00 mL of a solution of a dye and transfer 2.00 mL of the solution to a 100.00 mL volumetric flask. After adding water to the 100.00 mL mark, you take 5.00 mL of that solution and again dilute to 100.00 mL. If you find the dye concentration in the final diluted sample is 0.000129 M, what was the dye concentration in the original solution? Concentration
Serial dilutions question: You begin with an original solution of a drug named 2-APB, which is...
Serial dilutions question: You begin with an original solution of a drug named 2-APB, which is dissolved in DMSO and has a concentration of 50mg/mL. How would you use serial dilutions to make 25µL of final solution of 2-APB in DMSO that has a concentration of 20ng/µL?
You prepared a set of standard solutions by serial dilutions. You started with a stock solution...
You prepared a set of standard solutions by serial dilutions. You started with a stock solution of bromocresol green with a concentration of 0.213 M. The following standard solutions were prepared as described below. Solution A: Stock solution of bromocresol green at a concentration of 0.213 M. Solution B: 25 mL of solution A was diluted to 50 mL in a volumetric flask. Solution C: 25 mL of solution B was diluted to 50 mL in a volumetric flask. Solution...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
1.
2.
Using the equation C1V1 = C2V2, calculate the volume (V1) of concentrated solution and the volume of water needed to dilute the following solutions: Concentration Volume (in mls) Concentration of Volume (in mls) of Volume (in mls) of diluted of desired available stock stock solution required of solvent solution desired solution (V2) solution (C1) to make the diluted required to (C2) solution (V1) achieve desired volume (V2-V1) 5 mg/ml 100 ml 1000 mg/ml 15% 10 ml 50% 0.250...
Provided materials 0.01 to 0.05 M stock solutions of red and yellow dyes Dyo [stock] 0.0319 M V to mix red V of stock 100 ml 1.00 ml 264 mL yellow 0.0196 M 253 mL Experimental Procedure Dilute stock solutions by adding 1.00 mL of stock to sufficient distilled water to provide a total volume of 1.00 L 1.00 ml aliquots of stock solutions of red (0.0319 M) and yellow (0.0196 M) dyes are separately diluted with distilled water to...
Suppose you have 100.00 mL of a solution of a dye and transfer 2.00 mL of the solution to a 100.00 mL volumetric flask. After adding water to the 100.00 mL mark, you take 5.00 mL of that solution and again dilute to 100.00 mL. If you find the dye concentration in the final diluted sample is 0.000129 M, what was the dye concentration in the original solution? Concentration
You prepared a set of standard solutions by serial dilutions. You started with a stock solution of bromocresol green with a concentration of 0.213 M. The following standard solutions were prepared as described below. Solution A: Stock solution of bromocresol green at a concentration of 0.213 M. Solution B: 25 mL of solution A was diluted to 50 mL in a volumetric flask. Solution C: 25 mL of solution B was diluted to 50 mL in a volumetric flask. Solution...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
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