You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100,...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
Homework Answers
colony count = 200
dilution = 1/5 x 1/5 x 1/100 x 1/10 = 1/25000
100µl of final dilution (1/25000) plated
100µl = 1/10ml
Therefore,
1/10 ml of 1/25000 dilution gives 200 CFU
0.1ml x 0.00004 dilution gives 200 CFU
0.000004 ml gives 200 CFU
1ml gives 200/0.000004 CFU
= 5 x 107 CFU
Concentration of original culture is 5 x 107 CFU/ml
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