Question

Imagine that during the serial dilutions/spread plate procedure that your lab partner chose the wrong micropipet to transfer samples during the dilution. As a result, he/she ended up transferring 10 µl instead of 100 µl of culture in the last three steps. Therefore, the 1/1,000, 1/10,000, and 1/100,000 dilutions were actually much more diluted than expected. (Don’t yell at your lab partner.) Based on your partner’s mistake a) recalculate the actual dilutions and b) recalculate the concentration of cells from your stock culture.

Answer 1